Identifikasi Keragaman Gen FSH Bagian Ekson 2 Menggunakan Enzim Restriksi TasI pada Sapi Pesisir

Tinda Afriani; Endang Purwati; James Hellyward; Jaswandi Jaswandi; Yurnalis Yurnalis; Mangku Mundana; Anna Farhana; Adisti Rastosari

doi:10.24014/jupet.v19i2.17228

Identifikasi Keragaman Gen FSH Bagian Ekson 2 Menggunakan Enzim Restriksi TasI pada Sapi Pesisir

Tinda Afriani, Endang Purwati, James Hellyward, Jaswandi Jaswandi, Yurnalis Yurnalis, Mangku Mundana, Anna Farhana, Adisti Rastosari

Abstract

ABSTRAK. Studi ini bertujuan untuk mengidentifikasi keragaman gen FSH bagian ekson 2 menggunakan enzim restriksi TasI pada sapi Pesisir. Sampel darah yang digunakan yaitu 78 sampel darah dengan dua macam yaitu 13 jantan serta 65 betina yang berumur 1-5 tahun dari sapi pesisir yang didapat dari BPTUHPT Padang Mengatas. Protocol genomic DNA Purification system Kit dari Promega digunakan untuk mengisolasi DNA sampel darah. Amplifikasi gen FSH dilakukan dengan menggunakan teknik PCR atau Polymerase Chain Reaction. Enzim TasI digunakan untuk meretriksi produk amplifikasi. Hasil penelitian gen FSH bagian exon 2 pada sapi pesisir yaitu diperoleh terdapat dua macam genotip. Dua macam genotip tersebut yaitu heterozigot (+/-) serta homozigot tidak terpotong (-/-), untuk jantan berturut turut yaitu 0,38 dan 0,62 beserta frekuensi alel (+) dan alel (-) yaitu 0,19 dan 0,81, sedangkan untuk betina yaitu berturut-turut 0,43 dan 0,57 beserta frekuensi alel (+) dan alel (-) yaitu 0,21 dan 0,79. Sebaran genotip gen FSH|TasI exon 2 pada populasi Sapi Pesisir sifatnya polimorfik (beragam) dengan frekuensi alel yaitu kurang dari 0,99.

Identification of Exon 2 FSH Gene Diversity Using TasI Restriction Enzymes in Coastal Cattle

ABSTRACT. This study aims to identify the diversity of the exon 2 FSH gene using the restriction enzyme TasI in Coastal cattle. The blood samples used were 78 blood samples of two types, namely 13 males and 65 females aged 1-5 years, from coastal cattle obtained from BPTUHPT Padang Atas. The protocol genomic DNA Purification System Kit from Promega was used to isolate DNA from blood samples. The amplification of the FSH gene was carried out using the PCR or Polymerase Chain Reaction technique. The TasI enzyme was used to restrict the amplification product. The study results on the exon two portion of the FSH gene in coastal cattle showed two types of genotypes. The two types of genotypes are heterozygous (+/-) and untruncated homozygous (-/-); for males, 0.38 and 0.62, respectively, and the frequencies of allele (+) and allele (-) are 0.19 and 0. .81, while for females, it was 0.43 and 0.57, respectively, and the frequencies (+) and allele (-) were 0.21 and 0.79. The FSH|TasI exon two gene genotype distribution in the Coastal Cattle population is polymorphic (various) with an allele frequency of less than 0.99.

Keywords: FSH Gene, Pesisir Cattle, TasI Enzyme

Keywords

Gen FSH, sapi Pesisir, enzim TasI

Full Text:

PDF (Bahasa Indonesia)

References

Afriani, T., M. P. Agusta, Yurnalis, F. Arlina, dan D. E. Putra. 2019. Estimasi dinamika populasi dan pembibitan sapi ppotong di Kecamatan Bayang Kabupaten Pesisir Selatan. Jurnal Peternakan Indonesia, 21(2) : 130-142.

Anam, K. 2010. Isolasi DNA Genom. Bioteknologi Sekolah Pascasarjana Institut Pertanian Bogor.

Arslan, K., B. Akyuz, O. K. Agaoglu. 2015. Investigation of STAT5A, FSHR and LHR gene polymorphisms in turkish indigenous cattle breeds (east anatollan red, south anatolian red, turkish grey, anatolian black and zavot). Genetika, 51(11): 1088-1095.

Becker, W. M., L. Kleinsmith dan J. Hardin. 2000. World of the Cell. Ed 4th. The Benjamin Publishing Company.

Dai, L., Z. Zhao, R. Zhao, S. Xiao, H. Jiang, X. Yue, .........J. Zhang. 2009. Effects of novel single nucleotide polymorphism of the FSH beta sub unit gene on semen quality and fertility in bulls. Anim Reprod Sci, 114: 14-22.

Faatih, M. 2009. Isolasi dan digesti DNA Kromosom. Jurnal Penelitian Sains dan Teknologi, 10(1): 61-67.

Fan, Q. R. and W. A. Hendrickson. 2005. Structure of human follicle stimulating hormone incomplex with its receptor. Nature, 433: 269–277.

Grigorova, M., K. Rull. dan M. Laan. 2007. Haplotype structure of fshb, the beta sub unit gene forfertility-associated follicle stimulating hormone: possible influence of balancing selection. Ann Hum Gen, 71: 18-28.

Irmawati. 2003. Perubahan keragaman genetik ikan kerapu tikus generasi pertama pad stok hatchery. Thesis. IPB: Bogor. PP. 35.

Ishak, A. B. L., C. Sumantri, R. R. Noor, and I. Arifiantini. 2011. Identification of polymorphism of FSH beta-subunit gene as sperm quality marker in Bali cattle using PCR-RFLP. J Indonesian Trop Anim Agric, 36(4): 221-227.

Javanmard, A., N. Asadzadeh, M. H. Banabazi. and J. Tavakolian. 2005. The allele and genotype frequencies of bovine pituitary-specific trancription factor and leptin genes in iranian cattle and buffalo population using PCR-RFLP. Iran J Biotechol, 3(2): 104-108.

Kim, K.E., D.F. Gordon and R.A. Maurer. 1998. Nucleotide sequence of the bovine gene for follicle-stimulating hormone beta-subunit. DNA 7(4): 227-233.

Kusuma, R., N. Sa’diyah dan Y. Nurmiaty. 2016. Keragaman fenotipe dan heritabilitas kedelai (Glycine max[L.] Merrill) generasi F5 hasil persilangan Wilis x B3570. Jurnal Penelitian Pertanian Terapan.15 (3): 200-207.

Liu, J.J., X.Q. Ran, S. Li, Y. Feng, J. F. Wang. 2009. Polimorphism in the first intron of follicle stimulating hormone beta gene in three Chinese pig breeds and two European pig breeds. J. Anim Rep Sci. 111: 369-375.

Mandayasa, I. W. G. 2007. Studi keragaman genetik populasi udang galah (marcobrachium rosenbergii) berdasarkan polimorfisme mitokondria DNA. Skripsi. Bogor: IPB.

Mulyani, Y., A. Purwanto. dan I. Nurruhwati. 2011. Perbandingan beberapa metode isolasi DNA untuk deteksi Dini Koi Herpes Virus (KHV) pada ikan mas (Cyprinus carpio L.). Jatinangor: Fakultas Perikanan dan Ilmu Kelautan, Universitas Padjajaran. Jurnal Akuatika Vol.II No. 1/Maret Tahun 2011.

Nei, M. And S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.

Prihadini, P. W., A. Primasari, M. Lutfi, D. Pamungkas, T. B. Wibowo. 2019. Identifikasi keragaman gen follicle stimulating hormone sub-unit beta (FSH-ß) pada tiga sumber daya genetik sapi lokal Indonesia. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner, 183-191.

Utomo, B., E. D. Putranto, and A. Fadholly. 2020. Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction. Veterinary World, 13: 879- 883.

Warwick, E. J., J. M. Astuti dan W. Hardjosubroto. 1994. Pemuliaan Ternak. Edisi V. Gadjah Mada University Press, Yogyakarta. Hal 45-97.

Xiaopeng, A. N., H. Dan, H. Jin-Xin, L. Guang, W. Ya-Na, L. Ling,........C.Y. Bin Yun. 2010. Polymorphism of exon 2 of β gene and its relationship with reproduction performance in two goat breeds. Agric. Sci.China. 6:889-886.

Yang, W. C. 2010. Polymorphisms in the 5 upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows. Anim Rep Sci, 119: 172-177.

Yuniarsih, P., Jakaria dan Muladno. 2011. Eksplorasi gen growth hormone exon 3 pada kambing peranakan etawah (PE), Saanen dan Pesa melalui teknik PCR-SSCP. Seminar Nasional Teknologi Peternakan dan Veteriner,Bogor.

Yurnalis. 2013. Polimorfisme gen hormon pertumbuhan pada sapi pesisir sumatera barat. Disertasi. Program Pasca Sarjana Universitas Andalas, Padang.

Yuwono, T. 2006. Teori dan Aplikasi Polymerase Chain Reaction. Andi Offset. Yogyakarta. 237 p.

DOI: http://dx.doi.org/10.24014/jupet.v19i2.17228