KUALITAS SEMEN CAIR SAPI SIMENTAL MENGGUNAKAN LARUTAN ISOTONIS KOMERSIAL PADA KONSENTRASI DAN LAMA PENYIMPANAN BERBEDA

Bayu Anke Mardian, Zumarni Zumarni, Anwar Effendi Harahap

Abstract


Artificial insemination (AI) is a technique to insert the sperm or semen of bull thawed and processed first into the genital tract of female methods and a special tool called insemination gun, efforts to optimize the management of the cement in order to obtain the quality of cement is optimal to do with the selection of diluent cement. One alternative materials cement diluent is an isotonic solution Commercial (LIK). This study aimed to examine the interaction between the concentration and duration of storage of diluent solution isotonic different to the quality of spermatozoa cow simental. This research was conducted at the Regional Technical Implementation Unit of Artificial Insemination Centres (UPTD BIB) Tuah Sakato, Payakumbuh. In April 2016. This study used a randomized block design (RAK) factorial design to Factor A (Concentration LIK) ie A1 = 100 ml Tris Egg Yolk, A2 = (60 ml) Tris Egg Yolk + (40 ml) LIK, A3 = (55 ml) Tris Egg Yolk + (45 ml) LIK, A4 = (50 ml) Tris Egg Yolk + (50 ml) LIK, and factor B (retention) is B1 = 0 day, B2 = 1, B3 = 2 day, days, B4 = 3 days. Each treatment consists of three replicates. Parameters measured were motility, percentage of survival, abrnormalitas, and MPU. These results indicate that an isotonic solution can be used as an alternative Commercial diluent with the best results on the percentage LIK 40 ml, but the effectiveness of the efficiency of storage use was only on the first day (24 hours).


Keywords


Catle simental; Commercial isotonic solution; motility; percentage of survival; abnormalities and Plasma Membrane Whole (MPU)

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References


Arifiantini, R. I dan T.L. Yusuf. 2010. Keberhasilan Penggunaan Tiga Pengencer dalam Dua Jenis Kemasan pada Proses Pembekuan Semen Sapi Frisien Holstein. http://cari-pdf.com/download. Diakses pada tanggal 3 Desember 2015.

Aurich, J. E., U. Schoneher. H. Hoppe and C. Aurich. 1997. Effect of Antioxidands on Motility and Membrane Integrity of Chilled-Stored Stallition Semen. Theriogenology. 48 : 185-192.

Bearden, H. J, and J. W Fuquay. 1984. Applied Animal Reproduction. 2nd edition. Reston Publishing Company, Inc. Virginia.

Damayanti, Y. 1991. Pengaruh Kadar Fruktosa dalam Pengencer Air Kelapa Muda, Aiar Siwalan dan Kombinasinya dengan Kuning Telur terhadap Kualitas Air Mani Ayam Buras. Skripsi. Fakultas Kedokteran Hewan Universitas Airlangga Siurabaya. Hal : 33-35.

Hardijantono, S., Suslowatii, T. Sardjito, T. Hernawati, dan T.W. Suprayogi. 2010. Inseminasi Buatan. Fakultas Kedokteran Hewan. Airlangga University Press. Surabaya.

Herdiawan, I. 2004. Pengaru Laju Penurunan Suhu dan Jenis Pengencer Terhadap Kualitas Semen Beku Domba Priangan. Balai Penelitian Ternak. Bogor. JITV, 9 (2) : 98-107.

Ichmy, C. 2010. Uji Persentase Motilitad dan Daya Hidup Spermatozoa Domba dengan Pengencer Campuran Larutan Isotonis Komersial dan Kuning Telur. Skripsi. Fakultas Kedokteran Hewan. Universitas Airlangga. Surabaya.

Jayendran, R. S., Van der Van., M. P. Pelaes., B. G. Crabo and L. J. D. Zaneveld. 1984. Development of an Assy to Assess the Functional Integrity of the Human Sperm Membrane and its Relationship on Other Semen Characteristic. J. Reprod. Fertil, 70: 219-228.

Kamal, A. Gubartallah, A. Ahmed, Amel, Bakhiet, dan A. Babiker. 2005. Comparative Studies on Reproductive Performance of Nubian and Saanen Buck Under the Climatic Conditions of Khaortum. Journal of Animal and Veterinary Advances, 4 (11) : 942-944.

Kaya A, N. Baspinar, C. Yilidiz, F. Kurtoglu, M.B. Atman and S. Haliloglu. 2000. Influence of the Seminal Plasma and Plasma Testosteron Levels in Rams. Revue Medicine Veteriner. Turkey. Volume 151, issue 12, pages 1143-1146

Maxwell, W.M.C and P.F. Watson, 1996. Resent progress in the preservation of ram semen. J. Amin. Reprod. Sci. 42:55-65.

Norman, H.D., R.L. Powel, J.R. Wrigh and CG. Sittler. 2003. Timeliness and effectiveness of progeny Testing Thorght Artificial Insemination. http:/jds.fas.org/cgi/content/abstract/86/4/1513. Diakses pada tanggal 18 November 2015.

Nursyam. 2007. Perkembangan Iptek Bidang Reproduksi Ternak Untuk Meningkatkan Produktifitas Ternak. http://www. scribd.com/doc/ 141993004/ IPTEK-REPRODUKSI-TERNAK. Diakses 30 November2015.

Pocari Sweat, 2015.Komposisi Pocari Sweat.http://www.pocarisweat.com.ph.html Diakses pada tanggal 1 Desember 2015.

Said. S, Gunawan M, Kaiin EM, Tappa B. 2005. Daya Tahan Sperma Cair Sapi Simental yang Disimpan Dalam Straw pada Tempertaur 50C. Pusat Penelitian Bioteknologi. LIPI. buletin peternakan 16: 8-73.

Shukla, M.K. 2011. App Lied Veterinary Andrologiy and Frozen Semen Technology New India Pubsling Ageney. Pitam Pura, New Delhi.

Sinha, S., B.C. Deka, M.K. Tamulu, and B.N. Borgohain. 1996. Effect Of Equilibration Period and Glicerol Level In Tris Extender Of Quality Of Frozen Goat Semen. Indian Vet. J. 69 : 1107-1110.

Solihati, N. dan P.Kune. 2009. Pengaruh Jenis Pengencer Terhadap Motilitas dan Daya Hidup Spermatozoa Semen Cair Sapi Simental. Universitas Nusa Candana. Kupang. http://pustaka.unpad.ac.id/. Diakses pada tanggal 1 Desember 2015.

Souhoka D. F., M. J. Matatula., W. Marlene Mesang-Nalley dan M. Rizal. 2009. Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan. Fakultas Peternakan, Universitas Nusa Cendana, Nusa Tenggara Timur. Jurnal Veteriner, Vol. 10 No. 3 : 135-142

Sugiarti, T., E. Triwulanningsih, p. Situmorang, R. G. Sianturi, dan D. A. Kusumaningrum. 2004. Penggunaan Katalase dalam Produksi semen dingin sapi. Prosiding Seminar Nasional Teknologi Peternakan Dan Vateriner, Bogor. Hal : 215-220.

Susilawati, T., Suyadi, Nuryadi, Isnaini, N, dan Wahyuningsih, S. 1993. Kualitas Semen Sapi Fries Holland dan Sapi Bali Pada Berbagai Umur dan Berat Badan. Laporan Penelitian. Fakultas Peternakan Universitas Brawijaya. Malang.

Toelihere, M.R. 1981. Fisiologi Reproduksi Pada Ternak. Penerbit Angkasa. Bandung. 327 hal.

Toelihere, M.R. 1993. Inseminasi Buatan Pada Ternak. Penerbit Angkasa. Bandung. 292 hal.




DOI: http://dx.doi.org/10.24014/jupet.v14i2.3676

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