Kandungan dan Kualitas Nutrisi Campuran Darah Sapi dan Limbah Pertanian yang Difermentasikan oleh Bacillus Amyloliquefaciens sebagai Pakan Broiler

R. F. Ramadhan, Wizna Wizna, Y. Marlida, Mirzah Mirzah, H. Supratman

Abstract


ABSTRAK. Campuran darah sapi dan limbah dapat dimanfaatkan sebagai pakan hal ini di karenakan kadar protein yang tinggi pada darah sapi dan ketersediaan yang melimpah sehingga sangat potensial untuk digunakan sebagai pakan. Penelitian ini bertujuan untuk mencari perbandingan campuran terbaik antara darah sapi dan limbah industry pertanian (ampas kelapa, bungkil inti sawit dan bungkil kelapa), waktu fermentasi (24, 72 dan 120 jam) yang difermentasi oleh Bacillus amyloliquefaciens. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 3 x 3 x 3 dengan 3 ulangan. Faktor 1 yaitu perbandingan level darah dan limbah pertanian terdiri  dari : A1 =  0,8:1; A2 = 1:1, A3 = 1:1,2 (v/v), faktor 2 yaitu jenis limbah (adsorben) terdiri dari: B1 = ampas kelapa, B2 = bungkil inti sawit, B3 = bungkil kelapa, dan faktor 3 yaitu lama fermentasi terdiri dari: C1 = 24 jam, C2 = 72 jam, C3 = 120 jam. Hasil penelitian ini menunjukkan level darah 1:1,2, ampas kelapa, dan lama fermentasi 120 jam memiliki kandungan protein, serat kasar, retensi nitrogen, kecernaan serat kasar dan energi metabolisme terbaik dibandingkan dengan campuran yang lain. Kesimpulan penelitian ini yaitu campuran darah sapi dengan ampas kelapa (1:1,2) yang difermentasi oleh Bacillus amyloliquefaciens selama 120 jam menghasilkan kualitas nutrisi, retensi nitrogen, kecernaan serat kasar, dan metabolisme energi terbaik dibandingkan dengan campuran darah dengan limbah pertanian lainnya.

Content and Nutritional Quality of Mixed Bovine Blood and Agricultural Waste Fermented by Bacillus Amyloliquefaciens as Broiler Feed

ABSTRACT. A mixture of bovine blood and agricultural waste that can be used as animal feed, this is due to the high protein content in cow blood and its abundant availability so it is potential to be used as animal feed. The first stage is to find out the best combination between blood and agricultural waste (coconut pulp, palm kernel cake and coconut cake), and fermentation time (24, 72 and 120 hours) which is fermented by Bacillus amyloliquefaciens. This study used a 3x3x3 factorial completely randomized design (CRD) with 3 replications. Factor 1 is the ratio of blood levels and agricultural waste consisting of: A1 = 0.8:1, A2 = 1:1, A3 = 1:1.2 (v / v), factor 2 is the type of waste (adsorbent) consisting of: B1 = coconut dregs, B2 = palm kernel meal, B3 = coconut cake, and factor 3, namely the fermentation time consisting of: C1 = 24 hours, C2 = 72 hours, C3 = 120 hours. The results of this study showed a blood level of 1:1.2, coconut pulp, and fermentation time of 120 hours had the best protein, crude fiber, nitrogen retention, crude fiber digestibility and metabolic energy compared to other mixtures. The conclusion of this study is that the mixture of bovine blood with coconut pulp (1: 1.2) fermented by Bacillus amyloliquefaciens for 120 hours resulted in the best nutritional quality, nitrogen retention, crude fiber digestibility, and energy metabolism compared to blood mixtures with other agricultural wastes.


Keywords


Darah sapi, limbah pertanian, fermentasi, bacillus amyloliquefaciens

References


Amodou, I., Mohamed, T., Kamara, Tidjani, A., Foh, M.B.K. & Guo-Wei, L. 2010. Physochemical and nutritional analysis of fermented soybean protein meal by Lactobacillus plantarum Lp6. World Journal Dairy and Food Science 5: 14-118

AOAC. 2005. Official Method of Analysis of the Association of Official Analitycal Chemists. 18th ed. Maryland: AOAC International. William Harwitz (ed). United States of America.

Das, M. P., L. Jeyanthi R., S. Sharmila, Anu, Ankita B., & Dhiraj Kumar. 2012. Identification and optimization of cultural condition for chitinase production by Bacillus amyloliquefaciens SM3. Jurnal of Chemical and Pharmaceutical Research, 2012, 4(11):4816-4821.

Ginindza M. M., Jones W. N, & D. Norris. 2017. Effect of dietary crude fibre level on intake, digestibility and productivity ofslow-growing indigenous Venda chickens aged one to 91 days. Indian J. Anim. Res., 5(6): 1073-1079.

Hidayati, S.G. 2011. Pengolahan Ampas Kelapa Dengan Mikroba Lokal Sebagai Bahan Pakan Ternak Unggas Alternatif di Sumatera Barat. Jur. Embrio (4) (1) (26 -36).

Ibrahim, A.D., Mukhtar, Sa’adat I., Ibrahim, M.N., Oke, M.A. & Ajijolakewu, A.K. 2012. Adonsonia digitata (Baobab) fruit pulp as substrate for Bacillus amyloliquefaciens Endoglucanase production.

Madigan M, Martinko J, Stahl D, & Clark D. 2012. Brock Biology of Microorganisms. Ed ke-13. New York: Pearson.

Makinde, O. A. & E B Sonaiya. 2011. Utilization of sun-dried maize offal with blood meal in diets for broiler chickens. Journal of Animal Sciences. Vol. 1. No. 3. 106-111.

Martono, Y., L. D Danriani, & Hartini. S. 2016. Pengaruh Protein terhadap Kandungan Protein dan Asam Amino Pada Tepung Gaplek yang Difortifiaksi TepungKedelai (Glycine max (L))

McDonald, P., R.A. Edwards, J.F.D. Greenhalgh, C.A. Morgan, L.A. Sinclair, & R.G. Wilkinson. 2010. Animal Nutrition. 7thEd. Prentice Hall, Pearson, Harlow, England, London, New York, Boston, San Fransisco, Toronto, Sydney, Tokyo, Singapore, Hong Kong, Seoul, Taipei, New Delhi, Cape Town, Madrid, Mexico City, Amsterdam, Munich, Paris, Milan.

Mirnawati., B. Sukamto, & V.D. Yunianto. 2013. Kecernan Protein, Retensi Nitrogen dan Massa Protein Daging Ayam Broiler yang diberi Ransum daun Murbei (Morus alba L.) yang difermentasi dengan Cairan Rumen. JITP Vol 3 no 1.

Murrad & Azzaz. 2010. Cellulase and Dairy Animal Feeding. Journal Biotechnology. 9(3): 238-258.

Nurhajati, T. & T. Suprapto. 2013. Penurunan serat kasar dan peningkatan protein kasar sabut kelapa (Cocos nucifera Linn) secara amofer dengan bakteri selulotik (Actinobacillus ML-08) dalam pemanfaatan limbah pasar sebagai sumber bahan pakan. J. Agroveteriner. 2 (1) : 1-11.

R.F. Ramadhan, C. Yang, Wizna, Y. Marlida & Mirzah. 2017. Effects of amino acid composition in a Bacillus amyloliquefaciens-fermented mixture of bovine blood and coconut pulp on growth performance, blood cholesterol of broilers. Int. J. Poult. Sci., CC: CC-CC.

R.F. Ramadhan, Wizna, Y. Marlida & Mirzah. 2016. Fermentation of blood meal with Bacillus amyloliquefaciens as broiler feeding. Asian J. Anim. Vet. Adv., 11: 840-846.

Sarasvati, S., B. Sujata, & S. Amita. 2014. Effects of Fermentation on Nutritional Quality of Prosopis JulifloraPods as Alternative Fish Feed. Res. J. Animal, Veterinary and Fishery Sci. Vol. 2(12), 1-7

Selvamohan, T., V. Ramadas, & T. A. Sathya. 2012. Optimization of Lipase Enzyme Activity Produced by Bacillus amyloliquefaciensIsolated from Rock Lobster PanlirusHomareus. International Journal of Modern EngineringReserch (IJMER).

Setiowati S., E. Sudjarwo, & A. A. Hamiyanti. 2014. The effect of blood meal addition in the feed to carcass and giblet percentages of quail.

Sibbald, I.R., 1975. The effect of level intake on metabolizable energi values measured with adult roogter. poultry Science 54: 1990-1998.

Sibbald, I.R., & M.S. Wolynetz. 1984. Relationship between apparent and true metabolizable energi and the effect of nitrogen correction. Poult Sci. 63: 1386-1399.

Sinurat AP, T. Purwadaria, & T. Pasaribu. 2013. Peningkatan Nilai Gizi Bungkil Inti SawitdenganPenguranganCangkang dan PenambahanEnzim. JITV Vol. 18 No 1 Th. 2013: 34-41.

Wizna, H. Muis, & A. Deswan. 2014. Pengaruh Dosis Inokulum dan Lama Fermentasi Campuran Dedak Padi dan Darah dengan Bacillus amyloliquefaciens terhadap Kandungan Serat Kasar, Kecernaan Serat Kasar, dan Energi Metabolisme. Jurnal Peternakan Indonesia. ISSN 1907-1760.

Qingkui J., G. Lia, T. Zhanga, H. Zhanga, Xiuhua Gaob, Xiumei Xinga, J. Zhaoa, & F. Yang. 2015. Effects of dietary protein level on nutrients digestibility andreproductive performance of female mink (Neovison vison) during gestation. Animal Nutrition 1 (2015) 65-69.

Hernandez, F., Lopez, M.J., Garcia, V., Martínez, S., Megias, M.D, & Madrid, J. 2011. Influence of cereal type and the inclusion ofsunflower meal as a source of additional dietary fibre on nutrient retention, growth performance and digestive organ size inbroilers from one to twenty-one days of age. Anim Feed Sci Technol.165: 251-257.

Lindberg, J.E. 2014. Fiber effects in nutrition and gut health in pigs. J Anim Sci Biotechnol.5:15.




DOI: http://dx.doi.org/10.24014/jupet.v18i1.12060

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