Oxidative Stress Status in Heat Shock Sheep Controlled Shearing and Ascorbyl Palmitate Administration

Ahmad Taofik; Muhammad Yusuf

doi:10.24014/jupet.v21i1.26951

Oxidative Stress Status in Heat Shock Sheep Controlled Shearing and Ascorbyl Palmitate Administration

Ahmad Taofik, Muhammad Yusuf

Abstract

Indonesia has very hot temperatures and high humidity, making it unfavorable for sheep whose covered with thick fur. Shearing enlarges and accelerates heat dissipation from the sheep's body, giving antioxidants that help reduce the negative effects of excessive free radical production. Ascorbic acid is an antioxidant but is easily decomposed when it enters the digestive system of ruminants. Ascorbyl palmitate (AP) is ascorbic acid in the form of an ester which is insoluble in water. The aim of this study was to analyze the effect of shearing and administration of AP antioxidants on oxidative stress conditions in Padjadjaran sheep subjected to heat shock. The shaving treatment consisted of not shaving (s0) and shearing (s1),and the treatment of giving the antioxidant ascorbyl palmitate consisted of not being given (a0) and being given (a1). Sheep were subjected to shearing, their hair was trimmed until ± 10 mm in length was left, and sheep as object of study were treated with ascorbyl palmitate at a dose of 400 mg head-1 day-1. The combination treatment was not shaved – not given the antioxidant ascorbyl palmitate (s0a0), only shaved (s1a0), only given the antioxidant ascorbyl palmitate (s0a1), and sheared – given the antioxidant ascorbyl palmitate (s1a1). Heat shock is given by exposing the sheep to the sun. The oxidative stress observed and analyzed was the profile of endogenous antioxidants consisting of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). The results showed that the combination of shearing and Ascorbyl Palmitate had a significant effect (p<0.05) on the levels of SOD, CAT and GSH-Px in Padjadjaran sheep receiving heat stress. The conclusion from this study, the shearing treatment and the administration of Ascorbyl palmitate, both independently and in combination, had a beneficial effect on Padjadjaran sheep.

Keywords

Ascorbyl palmitate, oxidative stress, padjadjaran sheep

Full Text:

PDF

References

Adenkola, A. Y., & Angani, M. T. (2017). Ascorbic Acid Supplementation Effect on Haematology and Oxidative Stress Parameters of Broiler Chicken during the Hot-Dry Season in Southern Guinea Savannah Askorbik Asit İlavesinin Güney Gine Savana ’ da Sıcak -Kurak Mevsimde Broylerlerde Hematolojik ve. Journal of Poultry Research, 14(1): 28–33.

Aktas, M. S., Kandemir, F. M., Kirbas, A., Hanedan, B., & Aydin, M. A. (2017). Evaluation of oxidative stress in sheep infected with Psoroptes ovis using total antioxidant capacity, total oxidant status, and malondialdehyde level. Journal of Veterinary Research (Poland), 61(2): 197–201. https://doi.org/10.1515/jvetres-2017-0025

Al-dawood, A. (2017). Towards heat stress management in small ruminants – a review. 17(1): 59–88. https://doi.org/10.1515/aoas-2016-0068

Bernabucci, U., Ronchi, B., Lacetera, N., & Nardone, A. (2002). Markers of Oxidative Status in Plasma and Erythrocytes of Transition Dairy Cows During Hot Season. Journal of Dairy Science, 85(9), 2173–2179. https://doi.org/10.3168/jds.S0022-0302(02)74296-3

Celi, P. (2011). Biomarkers of oxidative stress in ruminant medicine. Immunopharmacology and Immunotoxicology, 33(2), 233–240. https://doi.org/10.3109/08923973.2010.514917

Chaudhary, S. S., Singh, V. K., Upadhyay, R. C., Puri, G., & Odedara, A. B. (2015). Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes. Veterinary World, 8(6), 727–731. https://doi.org/10.14202/vetworld.2015.727-731

Chauhan, S. S., Celi, P., Fahri, F. T., Celi, P., & Dunshea, F. R. (2014). Dietary antioxidants at supranutritional doses modulate skeletal muscle heat shock protein and inflammatory gene expression in sheep exposed to heat stress. Journal of Animal Science, 92(11), 4897–4908. https://doi.org/10.2527/jas2014-8047

Chetia, M., Sarma, S., Nath, R., & Tamuly, S. (2017). Studies on the seasonal variations in the antioxidant enzymes in Indian zebu cattle. Journal of Entomology and Zoology Studies, 5(2), 1362–1365.

Costa, I. C. R., Sutili, F. K., Da Silva, G. V. V, Leite, S. G. F., Miranda, L. S. M., & De Souza, R. O. M. A. (2014). Lipase catalyzed ascorbyl palmitate synthesis under microwave irradiation. Journal of Molecular Catalysis B: Enzymatic, 102, 127–131. https://doi.org/10.1016/j.molcatb.2014.02.002

Filho, A. E., Teodoro, S. M., Chaves, M. A., Eduardo Ferreira Dos Santos, P., da Silva, M. W. R., Murta, R. M., de Carvalho, G. G. P., & de Souza, L. E. B. (2011). Zona de conforto térmico de ovinos da raça Santa Inês com base nas respostas fisiológicas. Revista Brasileira de Zootecnia, 40(8), 1807–1814. https://doi.org/10.1590/S1516-35982011000800026

Ganaie, A. H., Shanker, G., Bumla, N. A., Ghasura, R. S., Mir, N. A., Sa, W., & Gb, D. (2013). Biochemical and Physiological Changes during Thermal Stress in Bovines. J Veterinar Sci Technol, 4(1), 1–6. https://doi.org/10.4172/2157-7579.1000126

Gesualdi, A., Sales, É. S. V., Freitas, R. S., da Costa Henry, F., de Oliveira, V. de P. S., & de Souza Gesualdi, A. C. L. (2014). Effects of heat stress on the physiological parameters and productivity of hair sheep in tropical and coastal environments. Revista Brasileira de Zootecnia, 43(10), 556–560. https://doi.org/10.1590/S1516-35982014001000008

Ghosh, M., Gupta, M., Kumar, R., Kumar, S., Balhara, A. K., & Singh, I. (2015). Relation between antioxidant status and postpartum anestrous condition in Murrah buffalo. Veterinary World, 8(10), 1163–1166. https://doi.org/10.14202/vetworld.2015.1163-1166

Ghosh, S., Singh, A. K., & Haldar, C. (2014). Adaptive and ecological significance of the seasonal changes in hematological, biochemical and hormonal parameters in the tropical goat Capra hircus. J Endocrinol Reprod, 17(2), 113–122.

Giorgio, D., Trana, A. Di, Gregorio, P. Di, Rando, A., Avondo, M., Bonanno, A., Valenti, B., & Grigoli, A. Di. (2020). Oxidative Status of Goats with Different CSN1S1 Genotypes Fed ad Libitum with Fresh and Dry Forages. Antioxidants, 9, 1–13. https://doi.org/10.3390/antiox9030224

Guo, J., Gao, S., Quan, S., Zhang, Y., Bu, D., & Wang, J. (2018). Blood amino acids profile responding to heat stress in dairy cows. 31(1), 47–53.

Hefnawy, A., Helal, M. A. Y., Sabek, A., & Shousha, S. (2018). Clinical, behavioral and biochemical alterations due to shearing stress in Ossimi sheep. Journal of Veterinary Medical Science, 80(8): 1281–1286. https://doi.org/10.1292/jvms.18-0150

Horváth, M., & Babinszky, L. (2019). Impact of selected antioxidant vitamins (Vitamin A , E and C ) and micro minerals ( Zn , Se ) on the antioxidant status and performance under high environmental temperature in poultry. A review. Acta Agriculturae Scandinavica, Section A — Animal Science, 0(0): 1–9. https://doi.org/10.1080/09064702.2019.1611913

Jaguezeski, A. M., Perin, G., Bottari, N. B., Wagner, R., Fagundes, M. B., C, M. R. S., Morsch, V. M., Stein, C. S., M, R. N., Barreta, D. A., Danieli, B., Defiltro, C. R., Schogor, A. L. B., & Silva, A. S. Da. (2018). Addition of curcumin to the diet of dairy sheep improves health, performance and milk quality. Animal Feed Science and Technology. https://doi.org/10.1016/j.anifeedsci.2018.10.010

Kizil, O., Ozdemir, H., Karahan, M., & Kizil, M. (2007). Oxidative stress and alterations of antioxi- dant status in goats naturally infected with Mycoplasma agalactiae. Revue Méd. Vét., 158(6): 326–330.

Lallawmkimi, M. C. (2009). Impact of Thermal Stress and Vitamin E Supplementation on Heat Shock Protein-72 and Antioxiddant Enzymes in Murrah Buffaloes. National Dairy Research Institute, 001: 1090603.

Lewandowska, A., Gierszewska, M., Marszalek, J., & Liberek, K. (2006). Hsp78 chaperone functions in restoration of mitochondrial network following heat stress. Biochimica et Biophysica Acta - Molecular Cell Research, 1763(2): 141–151. https://doi.org/10.1016/j.bbamcr.2006.01.007

Maan, R., Kataria, N., Pilania, P. K., Sharma, A., Laxmi, N. S., & Kataria, A. K. (2013). Fluctuations of serum glutathione reductase activities due to changes in extreme ambient temperatures in Marwari sheep from arid tracts. ELBA Bioflux, 5, 9–13.

Madene, A., Jacquot, M., Scher, J., dan Desobry, S. (2006). Flavour encapsulation and controlled release - A review. International Journal of Food Science and Technology, 41(1): 1–21. https://doi.org/10.1111/j.1365-2621.2005.00980.x

McManus, C., Paludo, G. R., Louvandini, H., Gugel, R., Sasaki, L. C. B., & Paiva, S. R. (2009). Heat tolerance in Brazilian sheep: Physiological and blood parameters. Tropical Animal Health and Production, 41(1): 95–101. https://doi.org/10.1007/s11250-008-9162-1

Megahed, G. A., Anwar, M. M., Wasfy, S. I., & Hammadeh, M. E. (2008). Influence of heat stress on the cortisol and oxidant-antioxidants balance during oestrous phase in buffalo-cows (Bubalus bubalis): Thermo-protective role of antioxidant treatment. Reproduction in Domestic Animals, 43(6): 672–677. https://doi.org/10.1111/j.1439-0531.2007.00968.x

Menéndez-Buxadera, A., Serradilla, J. M., Arrebola, F., Clemente, I., Castro, J. A., Osorio, J., Torres, R., & Molina, A. (2014). Genetic variation for tolerance to heat stress in dairy small ruminants : Results obtained in Spain Genetic variation for tolerance to heat stress in dairy small ruminants : Results obtained in Spain (Issue June 2013).

Miyazaki, T., Sueoka, K., Dharmarajan, A. M., Atlas, S. J., Bulkley, G. B., & Wallach, E. E. (1991). Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary. Journal of Reproduction and Fertility, 91(1): 207–212. https://doi.org/10.1530/jrf.0.0910207

Nandi, A., Yan, L., Jana, C. K., & Das, N. (2019). Review Article Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases. Oxidative Medicine and Cellular Longevity, 2019, 1–15.

Nazifi, S., Ghafari, N., Jahromi, I. ., & Razavi, M. (2009). Reference values of oxidative stress parameters in adult native Iranian goats. Bulgarian Journal of Veterinary Medicine, 12(2): 119–124.

Nazifi, S., Saeb, M., Baghshani, H., & Saeb, S. (2009). Influence of road transportation during hot summer conditions on oxidative status biomarkers in Iranian dromedary camels (Camelus dromedarius). African Journal of Biochemistry Research, 3(7): 282–287. http://www.academicjournals.org/AJBR

Nazifi, S., Saeb, M., Ghafari, N., Razeghian, I., Razavi, S. ., Vosoughi, F., Dehghani, F., & Orangi, H. (2009). Reference values of oxidative stress parameters in adult Iranian fat-tailed sheep. Pakistan Veterinary Journal, 30(1): 13–16.

Nwunuji, T. P., Mayowa, O. O., Yusoff, S. M., Bejo, S., Salisi, S., Abd, E., & Mohd, W. (2014). The ameliorative effect of ascorbic acid on the oxidative status , live weight and recovery rate in road transport stressed goats in a hot humid tropical environment. Animal Science Journal, 85 (June 2013): 611–616. https://doi.org/10.1111/asj.12174

Onmaz, A. C., Hoven, R. Van Den, Gunes, V., Cinar, M., & Kucuk, O. (2011). Oxidative stress in horses after a 12-hours transport period. Revue Méd. Vét., 162(4): 213–217.

Pandey, N., Kataria, N., Kataria, A. K., Joshi, A., Sankhala, N. La., Asopa, S., & Pachaury, R. (2012). Extreme ambiences vis-à-vis endogenous antioxidants of Marwari goat from arid tracts in India. Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society, 4(2): 29–33. http://www.doaj.org/doaj?func=fulltext&aId=936460

Piccione, G., Casella, S., Fazio, F., & Pennisi, P. (2008). Effect of shearing on some haematochemical parameters in ewes. Czech Journal of Animal Science, 53(3): 106–111.

Piccione, G., Fazio, F., Casella, S., Pennisi, P., & Caola, G. (2011). Influence of shearing on oxidative stress and some physiological parameters in ewes. Animal Science Journal, 82: 481–485. https://doi.org/10.1111/j.1740-0929.2010.00865.x

Prajoga, S. B. K., Damayanti, T., Darodjah, S., & Muchtar, R. (2015). Increased of Lamb Production on Padjadjaran Sheep through the Application of Syncronization Estrus and Artificial Insemination Innovation. Proceeding Oft 14th IInernational Conference on QIR (Quality in Research), 1059–1064.

Rathwa, S. D., Vasava, A. A., Pathan, M. M., Madhira, S. P., Patel, Y. G., & Pande, A. M. (2017). Effect of season on physiological, biochemical, hormonal, and oxidative stress parameters of indigenous sheep. Veterinary World, 10(6): 650–654. https://doi.org/10.14202/vetworld.2017.650-654

Rolf, M. M. (2015). Genetic Basis for Heat Tolerance in Cattle. Cab, March, 1–21. http://www.cabpartners.com/articles/news/2909/HeatToleranceCattle.pdf

Rout, P. K., Kaushik, R., Ramachandran, N., & Jindal, S. K. (2018). Identification of heat stress-susceptible and -tolerant phenotypes in goats in semiarid tropics. Animal Production Science, 58(7): 1349–1357. https://doi.org/10.1071/AN15818

Silanikove, N. (2000). Effects of heat stress on the welfare of extensively managed domestic ruminants. Livestock Production Science, 67(1–2): 1–18. https://doi.org/10.1016/S0301-6226(00)00162-7

Silanikove, N., Leitner, G., Merin, U., & Prosser, C. G. (2010). Recent advances in exploiting goat ’ s milk : Quality , safety and production aspects ଝ. Small Ruminant Research, 89(2–3): 110–125. https://doi.org/10.1016/j.smallrumres.2009.12.033

Sivakumar, A. V. N., Singh, G., dan Varshney, V. P. (2010). Antioxidants supplementation on acid base balance during heat stress in goats. Asian-Australasian Journal of Animal Sciences, 23(11): 1462–1468. https://doi.org/10.5713/ajas.2010.90471

Špiclin, P., Gašperlin, M., & Kmetec, V. (2001). Stability of ascorbyl palmitate in topical microemulsions. International Journal of Pharmaceutics, 222(2): 271–279. https://doi.org/10.1016/S0378-5173(01)00715-3

Steel, R.G.D., Torrie, J.H. and Dicky, D.A. (1997) Principles and Procedures of Statistics, A Biometrical Approach. 3rd Edition, McGraw Hill, Inc. Book Co., New York.

Sunil, K. B. ., Ajeet, K., & Meena, K. (2011). Effect of Heat Stress in Tropical Livestock and Different Strategies for Its Amelioration. Journal of Stress Physiology & Biochemistry, 7(1): 45–54.

Treichel, H., de Oliveira, D., Mazutti, M. A., Di Luccio, M., & Oliveira, J. V. (2010). A review on microbial lipases production. Food and Bioprocess Technology, 3(2): 182–196. https://doi.org/10.1007/s11947-009-0202-2

Weydert, C. J., & Cullen, J. J. (2010). Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nature Protocols, 5(1): 51–66. https://doi.org/10.1038/nprot.2009.197.

Winarno, G.D., Harianto, S.P. & Santoso, R. 2019. Klimatologi Pertanian. Pustaka Media. Bandarlampung.

DOI: http://dx.doi.org/10.24014/jupet.v21i1.26951