The Effect of Time and Dose from Gamma Irradiation (Cesium-137) on Protein Levels and Meat Color Index of Cork Fish (Channa Striata)

Miftahul Janna, Nurul Fuadi, Jumardin Jumardin, Sahara Sahara, Dwi Febri Isradianti, Serli Hatul Hidayat

Abstract


The study aimed to determine the effect of time and dose of gamma-ray irradiation on protein levels and storability based on the red color index of cork fish (channa striata). The results showed that the longer the irradiation time and the larger the dose given, the higher the protein level and absorbance value produced. The method used was UV-Vis spectrophotometry to determine protein levels and Image-J software to see the shelf life based on the red colour index. The sample consisted of control, irradiated with irradiation time of 35 minutes (6 mGy), 40 minutes (7 mGy) and 45 minutes (8 mGy) and an irradiation distance of 150 cm. The protein levels at control time, which were recorded at 35, 40, and 45 minutes, were 0.138%, 0.187%, 0.276%, and 0.347%, respectively. These results suggest that there may be a correlation between time and protein levels. Further analysis may be needed to fully understand the implications of these findings. The percentage close to stable on the first day with percentages of 1.54%, 1.66%, 1.76%, 2.50%. Additionally, it seems that a higher dose of irradiation may also result in a higher protein content and absorbance value.

Keywords: Cesium-137, Cork Fish, Irradiation, Protein, UV-Vis Spectrophotometer

 


Full Text:

PDF

References


Warid, N. Khumaida, A. Purwito, and M. Syukur, “Pengaruh Iradiasi Sinar Gamma pada Generasi Pertama (M1) untuk Mendapatkan Genotipe Unggul Baru Kedelai Toleran Kekeringan,” 2017.

B. Dwiloka. “Bahan Kuliah: Iradiasi Pangan, Fakultas Teknologi Pertanian, Universitas Semarang,”2002.

Z. Irawati, “Perkembangan Dan Prospek Proses Radiasi Pangan Di Indonesia,” no. 2, 2008.

I. N. Rahma. “Kajian Dan Analisis Pelat Timbal (Pb) Bekas Tutup Instalasi Listrik Pada Atap Rumah Sebagai Bahan Proteksi Radiasi Sinar-X,” Skripsi, 2016.

J. Ryan et al., “Microbial, physico‐chemical and sensory characteristics of mango juice‐enriched probiotic dairy drinks,” Int. J. Dairy Technol., vol. 73, no. 1, pp. 182–190, Feb. 2020, doi: 10.1111/1471-0307.12630.

E. M. Sari and T. M. Fadhilah “Penentuan Kadar Protein Albumin dalam Sampel Brownies yang Diberikan Kepada Penderita Tuberkulosis,” Chim. Nat. Acta, vol. 9, no. 2, Aug. 2021, doi: 10.24198/cna.v9.n2.34096.

D. González-Morales, A. Valencia, A. Díaz-Nuñez, M. Fuentes-Estrada, O. López-Santos, and O. García-Beltrán, “Development of a Low-Cost UV-Vis Spectrophotometer and Its Application for the Detection of Mercuric Ions Assisted by Chemosensors,” Sensors, vol. 20, no. 3, p. 906, Feb. 2020, doi: 10.3390/s20030906.

Widya Pangestika et al., “Pengaruh Iradiasi Gamma dan Penyimpanan Dingin terhadap Kandungan Proksimat, pH, dan ALT Filet Ikan Jenaha,” J. Pengolah. Has. Perikan. Indones., vol. 25, no. 1, pp. 80–87, Apr. 2022, doi: 10.17844/jphpi.v25i1.38521.

S. Riebroy, S. Benjakul, W. Visessanguan, M. Tanaka, U. Erikson, and T. Rustad, “Effect of irradiation on properties and storage stability of Som-fug produced from bigeye snapper,” Food Chem., vol. 103, no. 2, pp. 274–286, Jan. 2007, doi: 10.1016/j.foodchem.2006.07.046.

A. Y. Nasution, E. Novita, O. Nadela, and S. P. Arsila, “Penetapan Kadar Protein Pada Nanas Segar Dan Keripik Nanas Dengan Metode Spektrofotometri UV-Vis Dan Kjehdahl,” JOPS J. Pharm. Sci., vol. 4, no. 2, pp. 6–11, Jun. 2020, doi: 10.36341/jops.v4i2.1349.

A. S. Rusdianto, A. E. Wiyono, and N. Tauvika, “Penentuan Tingkat Kesegaran Daging Ayam Menggunakan Label Pintar Berbasis Ekstrak Antosianin Ubi Jalar Ungu,” J. Agroindustri, vol. 11, no. 1, pp. 11–22, May 2021, doi: 10.31186/j.agroindustri.11.1.11-22.

Jumardin“ Sintesis Nanopartikel Carbon (C-Dot) dengan Metode Ablasi Laser Untuk Aplikasi Bio-Imaging,” Tesis, 2017.

S. R. Manohara, S. M. Hanagodimath, and L. Gerward, “Energy absorption buildup factors of human organs and tissues at energies and penetration depths relevant for radiotherapy and diagnostics,” J. Appl. Clin. Med. Phys., vol. 12, no. 4, pp. 296–312, Sep. 2011, doi: 10.1120/jacmp.v12i4.3557.

A. B. D. Nandiyanto, R. Ragadhita, and M. Aziz, “How to Calculate and Measure Solution Concentration using UV-Vis Spectrum Analysis: Supporting Measurement in the Chemical Decomposition, Photocatalysis, Phytoremediation, and Adsorption Process”.

J. Nehete, M. Narkhede, R. Bhambar, and S. Gawali, “Natural proteins: Sources, isolation, characterization and applications,” Pharmacogn. Rev., vol. 7, no. 14, p. 107, 2013, doi: 10.4103/0973-7847.120508.

N. J. Anthis and G. M. Clore, “Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm: Sequence-Specific Protein Concentration at 205 nm,” Protein Sci., vol. 22, no. 6, pp. 851–858, Jun. 2013, doi: 10.1002/pro.2253.

P. Zhou, W. Yang, M. Li, and W. Wang, “A New Coupled Elimination Method of Soil Moisture and Particle Size Interferences on Predicting Soil Total Nitrogen Concentration through Discrete NIR Spectral Band Data,” Remote Sens., vol. 13, no. 4, p. 762, Feb. 2021, doi: 10.3390/rs13040762.

T. Surya, Y. Yulkifli, C. Imawan, N. Pratiwi, and D. Rahmawati, “Detektor Dosis Radiasi Gamma Menggunakan Prinsip Localized Surface Plasmon Resonance (LSPR) Nanopartikel Perak,” Sainstek J. Sains Dan Teknol., vol. 12, no. 2, p. 44, Dec. 2020, doi: 10.31958/js.v12i2.2521.

G. Martemucci, C. Costagliola, M. Mariano, L. D’andrea, P. Napolitano, and A. G. D’Alessandro, “Free Radical Properties, Source and Targets, Antioxidant Consumption and Health,” Oxygen, vol. 2, no. 2, pp. 48–78, Apr. 2022, doi: 10.3390/oxygen2020006.

M. Hasanah, “Pengaruh Iradiasi Sinar Gamma Terhadap Kadar Protein, Lemak Dan Radikal Bebas Daging Sapi (Bos Taurus),” Skripsi, 2019.

J. A. Reisz, N. Bansal, J. Qian, W. Zhao, and C. M. Furdui, “Effects of Ionizing Radiation on Biological Molecules—Mechanisms of Damage and Emerging Methods of Detection,” Antioxid. Redox Signal., vol. 21, no. 2, pp. 260–292, Jul. 2014, doi: 10.1089/ars.2013.5489.




DOI: http://dx.doi.org/10.24014/sitekin.v22i1.25387

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 SITEKIN: Jurnal Sains, Teknologi dan Industri



Editorial Address:
FAKULTAS SAINS DAN TEKNOLOGI
UIN SULTAN SYARIF KASIM RIAU
Kampus Raja Ali Haji
Gedung Fakultas Sains & Teknologi UIN Suska Riau
Jl.H.R.Soebrantas No.155 KM 18 Simpang Baru Panam, Pekanbaru 28293
Email: sitekin@uin-suska.ac.id
© 2023 SITEKIN, ISSN 2407-0939

SITEKIN Journal Indexing:

Google Scholar | Garuda | Moraref | IndexCopernicus | SINTA


Creative Commons License
SITEKIN by http://ejournal.uin-suska.ac.id/index.php