PENGARUH PENGENCER EKSTRAK AIR TEBU DENGAN PENAMBAHAN KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI
Abstract
This study aims to determine the motility and livability of the Bali bull semen with filtered sugarcane juice and the addition of egg yolk and preserved at a temperature of 50C. Semen used in this study was taken from four tested quality semen from Bali bull. The components of the extender used in this study are ; filtered sugarcane juice, egg yolk, distilled water and the antibiotic Streptomycin and penicillin. The material used in this study were ; four selected Bali cattle which were observed to have quality semen ; the extender used consisted of extracted sugar cane juice, egg yolks, distilled water and antibiotics. A completely randomized design (CRD) was used consisted of four treatments and 4 replications. The treatments applied were as follows ; T1 (10% extracted sugar cane juice + 20% egg yolk + 70% distilled water); T2 (15% extracted sugar cane juice + 20% egg yolk + 65% distilled water); T3 (20% extracted sugar cane juice + 20% egg yolk + 60% distilled water); T4 (25% extracted sugar cane juice + 20% egg yolk + 55% distilled water).The results showed that there were no significant effect (P>0.05) between treatments under different extender concentration levels and preservation days at a temperature of 50C for motility and livabilitas. Based on the results of this study, it can be concluded that the quality of Bali cattle’s liquid semen during the six days of preservation at 50C is above the average of 40% for the motility and livability. Among the various treatments the concentration of 25% extracted sugar cane juice with the addition of 20% egg yolk (T4) produced the best result.
Keywords
Full Text:
PDF (Bahasa Indonesia)References
Aisen EG., H. L. Alvarez., A. Venturino., dan J.
J. Garde. 2000. Effect of Trehalose and EDTA on Cryoprotective Action of Ram Semen Diluents. Theriogenology. 53:1053-
Aisen. E.G., H.L. Alvarez., dan A. Venturino.
Cryopreservation and Post Thawed Fertility of Ram Semen Frozen In Different Trehalose Concentration. Theriogenology
: 1801-1808.
Anwar, P. 2011. Motilitas dan Viabilitas Semen Sapi Bali yang Diencerkan dengan Pengenceran Air Tebu yang Berbeda. Skripsi. Fakultas Pertanian dan Peternakan Universitas Islam Negeri Sultan Syarif Kasim Riau. Pekanbaru.
Arifiantini, RI., T. Wresdiyanti., dan EF.
Retnani. 2006. Kajian Bandingan
Morfometri Spermatozoa Sapi Bali
(Bos Sondaicus) Menggunakan Perwarnaan Williams, Eosin, Eosin Nigrosin dan Formol Saline. J.Sain Vet. 24 (1): 65-70.
Bardan, Feradis, dan T. Adelina. 2009.
Penggunaan Air Tebu yang dikombinasikan dengan Kuning Telur sebagai Pengencer Semen Sapi Bali. Jurnal Peternakan, 6(2) : 37-44.
Bucak. M. N., dan N. Tekin. 2007. Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research 73: 103–108
Erwinda, M. D., dan Wahono. H. S. 2014. The Effect of Lime Concentration Addition and Cane Juice pH Value on Brown Sugar Quality. Jurnal Pangan dan Agroindustri.
(3) : 54 - 64.
Herdis., M.R. Toelihere., I. Supriatna,. B.
Purwantara, dan RTS. Adikara. 2005.
Optimalisasi Kualitas Semen Cair Domba Garut (Ovis Aries) Melalui Penambahan Maltosa ke dalam Pengencer Semen Tris Kuning Telur. Jurnal Media Kedokteran Hewan. 21 (2): 88-93
Hu. J.H., L. S. Zan., X. L. Zhao., Q. W. Li., Z. L.
Jiang., Y. K. Li., and X. Li. 2010. Effects of Trehalose Supplementation on Semen Quality and Oxidative Stress Variables in Frozen Thawed Bovine Semen. J Anim Sci,
: 1657-1662.
Jian. H.H,. Q. W. Li,. L. S. Zana,. Z. L. Jianga,. J.
H. Ana, L. Q. Wanga,. dan Y. H. Jia. 2010.
The Cryoprotective Effect of Low-Density Lipoproteins in Extenders on Bull Spermatozoa Following Freezing–Thawing. Animal Reproduction Science 117: 11–17.
Junior. A.S. V., C.D. Corcini., R.R. Ulguim., M.V.F. Alvarenga, I. Bianchi,. M.N. Corrêa., T. Lucia Jr., J.C. Deschamps. 2009. Effect of Low Density Lipoprotein on The Quality of Cryopreserved Dog Semen. Animal Reproduction Science 115: 323–327
Kusumaningrum. D.A., E. Triwulanningsih., P. Situmorang., T. Sugiarti., dan R.G. Sianturi. 2004. The Effect of Seminalo on the Quality of Diluted Semen Stored at Room
Temperature. Seminar Nasional Teknologi
Peternakan dan Veteriner : 207-213.
Labetubun J., dan I.P. Siwa. 2007. Kualitas Spermatozoa Kauda Epididimis Sapi Bali dengan Penambahan Laktosa atau Maltosa yang Dipreservasi pada suhu 3-5oC. Jurnal Veteriner. 12 (3) : 200-207.
Moussa M,. V. Marinet,. A. Trimeche,. D.
Tainturier,. Dan M. Anton. 2002. Low
Density Lipoproteins Extract From Hen Egg Yolk by an Easy Method: Cryoprotective Effect on Frozen-Thawed Bull Semen. Theriogenology 57: 1695–1706.
Munoz. O. V,. L. A. Briand,. T. Diaz,. L. V.
Squez., E. Schmidt., S. Desherces., M.
Anton., D. Bencharif., dan D. Tainturier.
Effect of Semen Dilution to Low-
Sperm Number Per Dose on Motility and Functionality of Cryopreserved Bovine Spermatozoa Using Low-Density Lipoproteins (LDL) Extender : Comparison To Triladyl1 And Bioxcell. Theriogenology
: 895–900.
Nalley. W. M. M., R. Hamdarini., dan B.
Purwantari. 2007. Viabilitas Spermatozoa
Rusa Timur (Cervus Timorensis) di dalam Pengencer Tris Kuning Telur dengan Penambahan Sumber Karbohidrat Berbeda yang Disimpan pada Suhu Ruang. JITV.
(4): 311-317.
Nainga. S.W., H. Wahida., K. M. Azamc., Y.
Rosninaa., A.B. Zukib., S. Kazhala., M.M. Bukara., M. Theind, T. dan, M.M. S. Kyawe.
Effect of sugars on characteristics of Boer goat semen after Cryopreservation. Animal Reproduction Science 122: 23–28.
Pramono. E., dan T. R. Tagama. 2005.
Pengaruh Penambahan Adenosin Triphospat ke dalam Pengencer Semen terhadap Kualitas Spermatozoa Domba Ekor Gemuk. Animal Production 10(3): 151-
Parerah, F., Z. Prihatiny., D.F. Souhoka, dan M. Rizal.2009. Pemanfaatan Sari Wortel sebagai Pengencer Alternatif Spermatozoa Epididimis Sapi Bali. J. Indon. Trop. Anim. Agric. 34 (1): 50-56.
Qomariyah. S. Mihardja., dan R. Idi. 2001. The Effect of Combination Egg Wolk with Coconut Water on Viability and Abnormality of Priangan Sheep Spermatozoa Stored at 50C. Seminar Nasional Teknologi Peternakan dan Veteriner : 173-178.
Reddy. N. S. S., G. J. Mohanarao., dan S.K.
Atreja. Effects of Adding Taurine and Trehalose To a Tris-Based Egg Yolk Extender on Buffalo (Bubalus Bubalis) Sperm Quality Following Cryopreservation. Animal Reproduction Science, 119:183–190.
Rizal M. 2009. Daya Tahan Spermatozoa Epididimis Sapi Bali yang Dipreservasi pada Suhu 3-50C dalam Pengencer Tris dengan Konsentrasi Laktosa yang Berbeda. JITV 14(2): 142-149.
Rizal M,. Herdis., A. Boediono., AS. Aku, dan Yulnawati. 2006. Peranan beberapa jenis gula dalam meningkatkan kualitas semen beku domba Garut. J Ilmu Ternak dan Veteriner. 11: 123-130.
Rizal M, Herdis, Yulnawati, dan H.
Maheshwari. 2007. Peningkatan kualitas spermatozoa epididimis kerbau belang yang dikriopreservasi dengan beberapa konsentrasi sukrosa. Jurnal Veteriner. : 188-
Steel, R.G.D, dan H.Torrie. 1995. Prisip dan Prosedur Statistik. Terjemahan; B.Sumantri. PT. Gramedia Pustaka Utama. Jakarta.
Surachman M., Herdisa., Yulnawati., M.
Rizalc., dan H. Maheshwari. 2008. Kualitas
Semen Cair Asal Epididimis Kerbau Belang dalam Bahan Pengencer Andromed yang Mendapat Penambahan Sukrosa. Media Peternakan.32 (2) : 88-94.
Silva. M.A,. G.C.X. Peixoto,. G.L. Lima,. J.A.B.
Bezerra,. L.B. Campos., A.L.C. Paiva,. V.V. Paula and A.R. Silva. 2012. Cryopreservation of collared peccaries (Tayassu tajacu) semen using a powdered coconut water (ACP-116c) based extender plus various concentrations of egg yolk and glycerol. Theriogenology 78: 605–611.
Tambing, S.N., K. Sutama dan M. Sariubang.
Efektivitas Konsentrasi Kuning Telur
di dalam Pengencer Tris dengan dan Tanpa Plasma Semen terhadap Kualitas Semen Beku Kambing Saanen. JITV 13(4): 315-322.
Toelihere, R.M. 1993. Inseminasi Buatan Pada
Ternak. Penerbit Angkasa Bandung
Widjaya, N. 2011. Pengaruh Pemberian Susu dengan Pengencer Tris Kuning telur Terhadap Daya tahan Hidup Spermatozoa Sapi pada suhu Penyimpanan 50C. Jurnal Sain Peternakan 9 (2) : 72-76.
Yulnawati,. M. Gunawan,. H. Maheshwari., M.
Risal., Herdis., dan A. Boediono. 2010. Qualiti of Epididimis and Ejaculated Sperms of Spotted Buffalo In Dextrose Suplemented Extender. J. Biosciences.
DOI: http://dx.doi.org/10.24014/jupet.v11i2.2719
Refbacks
- There are currently no refbacks.
Jurnal Peternakan has been accredited by Sinta 3 : Number 158/E/KPT/2021
Starting from Vol. 18 No. 1 Year 2021 to Vol. 22 No. 2 Year 2025
Jurnal Peternakan Indexed By:
Editorial Office:
Jurnal Peternakan
Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau.
H.R. Soebrantas street KM. 15,5 Panam – Pekanbaru city.
E-mail: jurnal.peternakan@uin-suska.ac.id
ejournal: http://ejournal.uin-suska.ac.id/index.php/peternakan
Creation is distributed under the Creative Commons Attribution 4.0 International License. View Mystats