PENGARUH WARNA LIGHT EMITTING DIODE (LED) TERHADAP MORFOGENESIS EKSPLAN KALUS ASAL BULBIL BAWANG PUTIH SANGGA SEMBALUN SECARA IN-VITRO

Sindi Ramadani; Nilla Kristina; Auzar Syarif; Elara Resigia

doi:10.24014/ja.v14i2.25211

PENGARUH WARNA LIGHT EMITTING DIODE (LED) TERHADAP MORFOGENESIS EKSPLAN KALUS ASAL BULBIL BAWANG PUTIH SANGGA SEMBALUN SECARA IN-VITRO

Sindi Ramadani, Nilla Kristina, Auzar Syarif, Elara Resigia

Abstract

The quality of the Garlic seed bulbs needs to be improved by seed propagation through tissue culture techniques to get uniform and big cloves. Utilizing the suitable color spectrum of light is one way to stimulate plant morphogenesis. This study aimed to identify the influence of light color and obtain the best color in the morphogenesis of garlic callus explants. This research was carried out from June to September 2022 at the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Andalas. This study used a completely randomized design with three treatment levels: red, blue, and white. The F test analyzed observational data at a 5% level, followed by Duncan's multiple Range Test (DNMRT) at a 5% level. The results showed light color influences embryogenic callus formation and shoot formation from garlic callus explants. Blue light is better than red and white light in inducing embryogenic callus, increasing the rate of explants forming shoots but giving no significant difference in the length of the shoot.

Keywords

LED, embryogenic callus, BAP, NAA

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References

Badan Pusat Statistik. (2022). Statistik Indonesia 2022. Badan Pusat Statistik.

Campbell Nail A. (2005). Biologi (8th ed., Vol. 1). Erlangga.

Devy, N. F., & Hardiyanto. (2009). Kemampuan regenerasi kalus segmen akar pada beberapa klon bawang putih lokal secara in vitro. J. Hort, 19(1), 6–13. https://doi.org/https://dx.doi.org/10.21082/jhort.v19n1.2009.p%p

Ermawati, D., Indradewa, D., & Trisnowati, S. (2012). The effect of additional light color on growth and flowering three varieties of Chrysanthemum cut flower plants (Chrysanthemum morifolium). Vegetalika, 1(3), 31–42. https://doi.org/https://doi.org/10.22146/veg.1354

Espinosa-Leal, C. A., Puente-Garza, C. A., & García-Lara, S. (2018). In vitro plant tissue culture: means for production of biological active compounds. Planta, 248, 1–18. https://doi.org/10.1007/s00425-018-2910-1

Fauziah, A., Widoretno, W., Biologi, J., Matematika, F., Ilmu, D., Alam, P., & Brawijaya, U. (2015). Regenerasi tanaman dari eksplan kalus bawang putih (Allium sativum L.) secara in vitro. In Jurnal Biotropika | (Vol. 3, Issue 1).

Fereol, L., Chovelon, V., Causse, S., Michaux-Ferriere, N., & Kahane, R. (2002). Evidence of a somatic embryogenesis process for plant regeneration in garlic (Allium sativum L.). Plant Cell Reports, 21(3), 197–203. https://doi.org/10.1007/s00299-002-0498-0

Hopkins, W. G., & Huner, N. P. A. (2009). Introduction to plant physiology (4th ed.). John Wiley & Sons, Inc.

Hurd, C. L., Harrison, P. J. (Paul J., Bischof, Kai., & Lobban, C. S. (2014). Seaweed ecology and physiology (2nd ed.). Cambridge University Press.

Ivanchenko, M. G., Napsucialy-Mendivil, S., & Dubrovsky, J. G. (2010). Auxin-induced inhibition of lateral root initiation contributes to root system shaping in Arabidopsis thaliana. Plant Journal, 64(5), 740–752. https://doi.org/10.1111/j.1365-313X.2010.04365.x

Kobayashi, K., Amore, T., & Lazaro, M. (2013). Light-Emitting Diodes (LEDs) for Miniature Hydroponic Lettuce. Optics and Photonics Journal, 03(01), 74–77. https://doi.org/10.4236/opj.2013.31012

Kristina, N., Yusniwati, Warnita, & Resigia, E. (2023). Growth and quality of seed bulb of four garlic varieties on different NPK level at Alahan Panjang, Indonesia. AIP Conference Proceedings, 2583. https://doi.org/10.1063/5.0116061

Lisiswanti, R., & Haryanto, F. P. (2017). Allicin in garlic (Allium sativum) as alternative therapy type 2 diabetes mellitus. Majority, 6(2), 31–36. https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/1009

Lizawati. (2012). Calli proliferation and somatic embryogenesis of physic nut (Jatropha curcas L.) various combination with PGR’s and amino acids. Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Jambi, 1(4), 256–265. https://online-journal.unja.ac.id/bioplante/article/view/1726

Lotfi, M. (2022). Effects of monochromatic red and blue light-emitting diodes and phenyl acetic acid on in vitro mass production of Pyrus communis “Arbi.” Journal Of Horticulture and Postharvest Research, 5(2), 119–128. https://doi.org/10.22077/jhpr.2021.4517.1229

Luz, J. M. Q., de Azevedo, B. N. R., Silva, S. M., de Oliveira, C. I. G., de Oliveira, T. G., de Oliveira, R. C., & Castoldi, R. (2022). Productivity and quality of garlic produced using below-zero temperatures when treating seed cloves. Horticulturae, 8(96). https://doi.org/10.3390/horticulturae8020096

Marbun, C. L. M., Toruan-Mathius, N., Reflini, Utomo, C., & Liwang, T. (2015). Micropropagation of embryogenic callus of oil palm (Elaeis Guineensis Jacq.) using temporary immersion system. Procedia Chemistry, 14(2015), 122–129. https://doi.org/10.1016/j.proche.2015.03.018

Martínez-Estrada, E., Caamal-Velázquez, J. H., Morales-Ramos, V., & Bello-Bello, J. J. (2016). Light emitting diodes improve in vitro shoot multiplication and growth of Anthurium Andreanum Lind. Propagation of Ornamental Plants, 16(1), 3–8. https://www.researchgate.net/publication/299583556

Meneses, A., Flores, D., Muñoz, M., Arrieta, G., & Espinoza, A. M. (2005). Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis. Revista de Biologia Tropical, 53(3–4), 361–368. https://doi.org/10.15517/rbt.v53i3-4.14598

Pardal, S. J. (2002). plantregeneration2002pdf. Buletin Agrobio, 5(2), 37–44.

Park, S. H., Elhiti, M., Wang, H., Xu, A., Brown, D., & Wang, A. (2017). Adventitious root formation of in vitro peach shoots is regulated by auxin and ethylene. Scientia Horticulturae, 226, 250–260. https://doi.org/10.1016/j.scienta.2017.08.053

Pramesti, R. (2013). Media air laut yang diperkaya terhadap laju pertumbuhan rumput Gracilaria Lichenoides (L) Harvey. Buletin Oseanografi Marina, 2, 66–73. https://doi.org/https://doi.org/10.14710/buloma.v2i1.6929

Ramírez-Mosqueda, M. A., Iglesias-Andreu, L. G., & Luna-Sánchez, I. J. (2017). Light quality affects growth and development of in vitro plantlet of Vanilla planifolia Jacks. South African Journal of Botany, 109, 288–293. https://doi.org/10.1016/j.sajb.2017.01.205

Saini, S., Sharma, I., Kaur, N., & Pati, P. K. (2013). Auxin: A master regulator in plant root development. In Plant Cell Reports (Vol. 32, Issue 6, pp. 741–757). https://doi.org/10.1007/s00299-013-1430-5

Santoso, J., Suhardjono, H., & Wattimury, A. (2020). The study of color spectrum curs value against sunlight color and artificial light for plant growth. NST Proceedings, 11–22. https://doi.org/10.11594/nstp.2020.0602

Syafriyudin, & Ledhe, N. T. (2015). Analisis pertumbuhan tanaman Krisan pada variabel warna cahaya lampu LED. Jurnal Teknologi, 8(1), 83–87. https://www.academia.edu/22934072/ANALISIS_PERTUMBUHAN_TANAMAN_KRISAN_PADA_VARIABEL_WARNA_CAHAYA_LAMPU_LED

Wahyuningtiyas, L., Resmisari, R. S., & Nashichuddin, A. (2014). Induksi kalus akasia (Acacia mangium) dengan penambahan kombinasi 2,4-D dan BAP pada media MS. Jurusan Biologi Fakultas Sains Dan Teknologi, 1–10. http://etheses.uin-malang.ac.id/376/12/10620033%20 Rangkuman.pdf

Wardana, S. T. (2016). Variasi Rasio Amonium dan Nitrat terhadap Perkembangan Embrio Somatik Bawang Putih (Allium sativum) secara In Vitro. In Jurnal Penelitian Sains (Vol. 18). https://doi.org/https://doi.org/10.56064/jps.v18i2.28

Widyawati, G. (2010). Pengaruh variasi konsentrasi NAA dan BAP terhadap induksi kalus jarak pagar (Jatropha curcas L.). http://eprints.uns.ac.id/4435/1/134720908201001581.pdf

Yelnititis. (2020). Embryogenic callus and somatic embryo induction from leaf explant of Kulim (Scorodocarpus borneensis Becc.). Jurnal Pemuliaan Tanaman Hutan, 14(2), 75–83. https://doi.org/https://doi.org/10.20886/jpth.2020.14.2.73-81

Yuniardi, F. (2019). Aplikasi Dimmer Switch pada rak kultur sebagai pengatur kebutuhan intensitas cahaya optimum bagi tanaman in vitro. Indonesian Journal of Laboratory, 2(1), 8–13. https://doi.org/https://doi.org/10.22146/ijl.v1i4.52991

DOI: http://dx.doi.org/10.24014/ja.v14i2.25211