Perbandingan Efek BAP dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

Camelia Andriani; Muhamad Kadapi; Erni Suminar; Anne Nuraini

doi:10.24014/ja.v14i1.18824

Perbandingan Efek BAP dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

Camelia Andriani, Muhamad Kadapi, Erni Suminar, Anne Nuraini

Abstract

Seed propagation of strawberry by in vitro culture is needed to obtain high quality seeds of strawberry in large quantities. In in vitro culture, a large number of shoots is expected can be produced at the multiplication stage. Cytokinins type of PGR can be added to culture media to produce a large number of shoot. The focus of this research was to compare the effect of BAP and Kinetin affected Sweet Charlie strawberry multiplication. The experiment was located at the Seed Technology Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University. The experimental design was a Completely Randomized Design (CRD) with two treatments of different types of cytokinin, namely Benzyl Amino Purine (BAP) and Kinetin at 1 mg L^-1 on Murashige and Skoog (MS) basic media, each treatment was repeated four times. This study showed that BAP had a better effect than Kinetin on the number of shoots, number of leaves, and wet weight of strawberry Sweet Charlie cultivar. The addition of BAP was recommended over Kinetin to obtain the highest number of shoots, the highest number of leaves, and the highest wet weight of culture on strawberry Sweet Charlie cultivar.

Keywords

kultur jaringan; pertanian; sitokinin; in vitro; ZPT; BAP; Kinetin; Multiplikasi; Stroberi

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References

Abu-Romman, SM, Al-Hadid, KA, & Arabiyyat, AR 2015, ‘Kinetin is the most effective cytokinin on shoot multiplication from cucumber’, Journal of Agricultural Science, vol. 7, no. 10, pp. 159-165, https://doi.org/10.5539/jas.v7n10p159.

Agustina, M, Maisura, M, & Handayani, RS 2020, ‘The effect of different seed cutting treatments and concentrations of BAP for the successful in vitro micrografting of mangosteen (Garcinia mangostana L.)’, Journal of Tropical Horticulture, vol. 3, no. 1, pp 1-5. https://doi.org/10.33089/jthort.v3i1.37

Amer, MS, Arisha, HME, Bardisi, A, & Nawar, DA, 2013. ‘In vitro study on strawberry shoot multiplication and rooting by using synthetic plant growth regulators in camparasion with moringa leaf extract’, Zagazig Journal of Agricultural Research, vol. 40, no. 6, pp. 1071-1082.

Ashrafuzzaman, M, Faisal, SM, Yadav, D, Khanam, D, & Raihan, F 2013, ‘Micropropagation of strawberry (Fragaria ananassa) through runner culture’, Bangladesh Journal of Agricultural Research, vol. 38, no. 3, pp. 467-472. https://doi.org/10.3329/bjar.v38i3.16973.

Avivi, S, Soedarmo, SH, & Prasetyo, PA 2013, ‘Multiplikasi tunas dan aklimatisasi tiga varietas pisang: Raja Nangka, Kepok, dan Mas’, Jurnal Hortikultura Indonesia, vol. 4, no. 2, pp. 83-89. https://doi.org/10.29244/jhi.4.2.83-89

Bakar, M, Mandang, J, Kojoh, D, & Demasabu, S 2016, ‘Penggunaan BAP dan Kinetin pada induksi tunas dari protocorm anggrek dendrobium (Dendrobium sp.) pada kultur in vitro’. Cocos, vol. 7, no. 4, pp. 1-6.

Balitjestro 2010, Mengenal Stroberi, diakses 25 November 2021, .

Bimantara, DS, Maghfoer, MD, Barunawati, N, Yenni, Y, & Siregar, AS 2018, ‘Multiplikasi kultur meristem stroberi kultivar earlibrite dengan penambahan konsentrasi hormon BAP dan Kinetin’, Jurnal Produksi Tanaman, vol. 6, no. 3, pp. 432-437.

Bozsó, Z, & Barna, B 2021, ‘Diverse effect of two cytokinins, kinetin and benzyladenine, on plant development, biotic stress tolerance, and gene expression’, Life, vol. 11, no. 12, pp 1404. https://doi.org/10.3390/life11121404

Costa, ADO, Silva, LAS, Duarte, IM, Sampaio, VF, Machado, M, Silva, GZD, & Rocha, DI 2019, ‘Kinetin and 6-benzyladenine induce different morphogenetic responses in cotyledonary segments of royal poinciana’, Ornamental Horticulture, vol. 25, pp. 270-275. https://doi.org/10.1590/2447536x.v25i3.2040

Davies, PJ 2010, The plant jormones: their nature, occurrence, and functions, in Davies, PJ (eds.), Plant hormones, Springer, Dordrech.

Erawati, DN, Mawaddah, Y, Humaida, S, & Wardati, I 2021, ‘Optimasi konsentrasi kinetin dan Benzyl Amino Purine pada kultur tunas vanili (Vanilla planifolia)’, Jurnal Ilmiah Inovasi, vol. 21, no. 1, pp. 54-57. https://doi.org/10.25047/jii.v21i1.2636.

Giampieri, F, Tulipani, S, Alvarez-Suarez, JM, Quiles, JL, Mezzetti, B & Battino, M 2012, ‘The strawberry: Composition, nutritional quality, and impact on human health’, Nutrition, vol. 28, no. 1, pp. 9–19.

Ibrahim, MSD & Hartati, RR 2014, ‘Multiplikasi tunas kopi arabika menggunakan Kinetin dan 6-benzylaminopurine’, Prosiding Seminar Nasional Tahun 2014, 1114-1123

Iliev, I, Gajdoov, A, Libiakov, G, & Jain, SM 2010, Plant micropropagation, in Davey, MR & Anthony, P (eds.), Plant cell culture: essential methods, Elsevier, UK.

Kalra, G & Bhatla, SC 2018, Cytokinins, Bhatla, SC & Lal, MA (eds.), Plant physiology, development and metabolism, pp. 603-615, Springer, Singapore.

Loyola-Vargas, VM & Ochoa-Alejo, N 2018, An introduction to plant tissue culture : advances, in Methods in molecular biology, Springer Science+Business Media, LLC.

Murgayanti, Fatilla, NR, & Sumadi 2020, ‘Peningkatan pertumbuhan tunas kunyit putih pada perbanyakan in vitro melalui aplikasi berbagai jenis dan konsentrasi sitokinin’, Kultivasi, vol. 19, no. 3, pp. 1230-1236. https://doi.org/10.24198/kultivasi.v19i3.29469.

Naing, AH, Kim, SH, Chung, MY, Park, SK, & Kim, CK 2019, ‘In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars’, Plant methods, vol. 15, no. 1, pp. 1-10. https://doi.org/10.1186/s13007-019-0421-0.

Praseptiana, C, Darmanti, S, & Prihastanti, E 2017, ‘Multiplikasi tunas tebu (Saccharumo officinarum L var. Bululawang) dengan perlakuan konsentrasi BAP dan kinetin secara in vitro’, Buletin Anatomi dan Fisiologi, vol. 2, no. 2, pp. 153-160. https://doi.org/10.14710/baf.2.2.2017.153-160.

Raisya, E, Sobarna, DS, Nuraini, A, Mubarok, S, Suminar, E, & Akutsu, M 2020, ‘Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit’, Jurnal Kultivasi, vol. 19, no. 3, pp. 1189-1195. https://doi.org/10.24198/kultivasi.v19i3.26932.

Rasud, Y, Ulfa, S, & Baharia, B 2015, ‘Pertumbuhan jeruk manis (Citrus sinensis L.) dengan penambahan berbagai konsentrasi sitokinin secara in vitro’, Agroland: Jurnal Ilmu-ilmu Pertanian, vol. 22, no. 3, pp. 197-204.

Sukmadjaja, D 2014, Pengadaan benih tanaman melalui teknik kultur jaringan, IAARD Press, Jakarta.

Yunus, A, Rahayu, A, Samanhudi, S, Pujiasmanto, B, & Riswanda, HJ 2016, ‘Respon kunir putih (Kaempferia rotunda) terhadap pemberian IBA dan BAP pada kultur in vitro’, Agrosains, vol.18, no. 2, pp. 44-49. https://doi.org/10.20961/agsjpa.v18i2.18690.

Zhou, J, Liu, Y, Wu, L, Zhao, Y, Zhang, W, Yang, G & Xu, Z 2021, ‘Effects of plant growth regulators on the rapid propagation system of broussonetia papyrifera l. Vent explants’, Forests, vol. 12, no. 7.

DOI: http://dx.doi.org/10.24014/ja.v14i1.18824