PERCEPATAN PENYEDIAAN BIBIT JERUK KUOK (Citrus nobilis Lour) DENGAN METODE KULTUR EMBRIO

Imam Mahadi

doi:10.24014/ja.v16i2.36574

PERCEPATAN PENYEDIAAN BIBIT JERUK KUOK (Citrus nobilis Lour) DENGAN METODE KULTUR EMBRIO

Imam Mahadi

Abstract

Kuok sweet oranges or Kuok oranges is derived from Siam oranges (Citrus nobilis Lour) is a local fruit Riau province crowded popular by the public. This fruit provides a promising business opportunity, however the limited availability of seedlings will need to be managed for the assessment of getting ready-to-be seeds available. Therefore, this study aims to determine the effect of IAA and Giberellin on the growth of embryo culture of Kuok oranges (Citrus nobilis Lour). This research uses Factorial Random Design. For the first factor Mikropropagasi IAA (I) consisted of 0 mgl-1 - 3 mgl-1 and the second actor Giberellin (G) consisted of 0 mgl-1 -4 mgl-1. The parameters measured were the time of shoots, the percentage of live eksplan, the time of the root, the number of shoots, the number of roots, and the shoot height. Data were analyzed using ANOVA and tested further with DNMRT at 5%. The results showed that the combination of IAA and Giberellin significantly affected the growth of plantlets from Kuok sweet organge embryos. The I2G3 treatment (2 mgL-1 IAA and 2 mgL-1 Giberellin) was the best for the average shoot growth time of the fastest 5,67 days after culture, 100% plantlet survival percentage, the average number of shoots was 5.83, the average shoot height was 10.67 cm, average root growth 7.83 days after culture and the average number of roots was 5.33. The plantlets from this study can be used as a source of germplasm for Kuok sweet orange seeds.

Keywords

Giberellin, Embryo Culture, Multiplication shoot, Plantlet

Full Text:

PDF

References

Agisimanto, D, Normah, MN & Ibrahim, R, 2019 ‘Rapid somatic embryogenesis of Citrus reticulata Blanco cv. Madu in An Air-Lift Bioreactor Culture’, AGRIVITA Journal of Agricultural Science. Vol. 41, no. 2, pp. 284-294.

Ahmed, AA, Bashi, AZK & Alwan, JM, 2023, ‘Micropropagation of three citrus rootstocks by tissue culture’ In IOP Conference Series: Earth and Environmental Science, vol. 1214, no. 1, pp. 12-22.

Balilashaki, Vahedi, & Karimi, 2015 ‘In Vitro direct regenaration from node and leaf explant of Phalaenopsis cv. Surabaya’, Plant Tissue Culture and Bitechnology, vol. 25, no. 2, pp. 193-205

Balitbag, 2012 Jendela Informasi Riau. (Online), http://www.riauounline.com/berita/print/balitbag-sukses-teliti-jeruk-carizzo-dan-siam-kampar.html (diakses 19 Desember 2014).

Cahyati, S, Isda, MN & Lestari, W 2016 ‘Induksi tunas dari eksplan kotiledon dan epikotil in vitro jeruk siam (Citrus nobilis Lour) asal kampar pada media MS’, Jurnal Riau Biologia, vol. 1, no. 5, pp. 31-38

Cengiz, M, & Kaçar, YA 2019, ‘Micropropagation of some citrus rootstocks with classical and new generation tissue culture techniques’, Turkish Journal of Agriculture-Food Science and Technology, vol. 7, no. 9, pp. 1469-1478. DOI: https://doi.org/10.24925/turjaf.v7i9.1469-1478.2846

Dian Pramita Wardani, Solichatun, Ahmad Dwi Setyawan, 2004, ‘Pertumbuhan dan produksi saponin kultur kalus talinum paniculatum gaertn pada variasi penambahan asam 2,4-Diklorofenoksi Asetat (2,4-D) dan kinetin’, Biofarmasi vol. 2 no. 1, pp. 35-43

Darnell, J, Lodish, H & Baltimore, H 1986, Molecular Cell Biology. Scientific American Books, Inc. New York.

Depdiknas. 2008, Panduan Pengembangan Bahan Ajar. Departemen Pendidikan Nasional, Direktorat Pembinaan Sekolah Menengah Atas, Jakarta.

Erniwitama, Taryono & Harimurti, R, 2018 ‘Effect of explant types and benzyl amino purine concentrations on the in vitro regeneration of several local eggplant cultivars’, Agrinova vol. 1 no. 1, pp. 1-7.

Fatonah S , Lestari W, Isda, MN & Purba, L, 2018 ‘In Vitro shoot regeneration of Citrus Nobilis Lour. from intact seed and cotyledon explants’, Sabrao Journal Of Breeding and Genetics, vol. 50, no. 2, pp. 168-179.

Firman, S, Mahadi, I & Darmawati, 2017 ‘Mikropropagasi In Vitro Jeruk Kuok (Citrus Nobilis Lour) Menggunakan Hormon 2,4-D dan TDZ sebagai Potensi Rancangan Modul Pembelajaran Biologi SMA’, JOM FKIP UNRI

Handayani, I, Nazirah, L & Handayani, SS, 2020 ‘The effect of BAP and IBA on in vitro root cultures of acehnes pomelo (Citrus maxima (Burm.) Merr.)’, Journal of Tropical Horticulture, vol. 3, no. 1, pp. 38-42.

Indah & Ermavitalini, 2013 ‘induksi kalus daun nyamplung (Calophyllum inophyllum Linn) pada beberapa kombinasi konsentrasi 6-benzylaminopurine (BAP) dan 2,4-diclorophenoxyacetit acid (2,4-D)’, Jurnal Sains dan Seni Pomits, vol. 2, no. 1, pp. 2337-3520.

Intarti, I, 2021 ‘Optimasi variasi zat pengatur tumbuh NAA (napthalnene aceticacid) dan BAP (benzylaminopurine) pada pembentukan plantet tanaman jeruk siam (Citrus nobilis var. Microcarpa) secara Invitro’, Borneo Journal of Science and Mathematics Education, vol. 1, no. 1, pp. 17-28.

Komairoh, S & Gusmalawati, D, 2023, ‘Respon pertumbuhan in vitro epikotil jeruk siam pontianak (Citrus nobilis L. var microcarpa) dengan penambahan ekstrak tomat dan benzylaminopurin (BAP)’, G-Tech: Jurnal Teknologi Terapan, vol. 7, no. 3, pp. 888-896.

Leupin, Ruth E, Leupin Mariane, Charles Ehret, Karl H. Erismann, and Witholt Bernard 2000, ‘Compact callus induction and plant regeneration of a non-flowering vitiver from java. plant cell, tissue and organ culture’, Switzerland, vol. 62, pp. 115-123

Lestari EG, 2011 ‘Peranan zat pengatur tumbuh dalam perbanyakan tanaman melalui kultur jaringan’, Jurnal AgroBiogen, vol. 7, no. 1, pp. 63-68.

Mahadi I, Syafi’i W, Sari Y, 2016 ‘Induksi kalus jeruk kasturi (Citrus microcarpa) menggunakan hormon 2,4-D dan BAP dengan metode in vitro’, Jurnal Ilmu Pertanian Indonesia (JIPI), vol. 21, no. 2 pp. 84-89.

Mahadi, I, Wulandari, S, Safii, W & Sayuti, I, 2021 ‘Kultur suspensi sel tanaman gajah beranak (Goniothalamus tapis Miq) terhadap kandungan zat goniotalamin, Jurnal Agro, vol. 8, no. 2, pp. 247-261.

Maryani, Y & Zamroni, 2005 ‘Penggandaan tunas krisan melalui kultur jaringan’, Ilmu Pertanian, vol. 12, no. 1, pp. 51-55

Mustika, T, Elis, T & Nicen, M, 2022 ‘Respon pertumbuhan biji jeruk keprok Citrus reticulata Blanco pada beberapa teknik sterilisasi’, Jurnal Ilmu Alam dan Lingkungan vol. 13, no. 2 pp. 32 – 39.

Nurul Hidayanti, Lestari, W, Isda MN, 2014 ‘Induksi tunas in vitro jeruk siam (Citrus nobilis Lour) asal kampar dari eksplan tunas apeks dan nodus in vitro, JOMFMIPA, vol. 1, no. 2, pp. 275-282

Prihastanti, E, Hastuti, ED & Suedy, SWA, 2020 ‘Short communication: comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods’, Biodiversitas, vol. 21, no. 12, pp. 5845-5849.

Robles-Martinez, M, Barba-de la Rosa, AP, Gueroud, F, Nigre-Salvayre, A, Rossognol, M &Santos-Diaz, MS, 2016 ‘Establishment of callus and cell suspensions of wild and domesticated Opuntia Species: Study on their potential as a source of metabolite production’, Plant Cell, Tissue and Organ Culture, vol. 124, no. 1, pp. 181-189.

Widia, SW, Mahadi, I & Darmawati, 2017 ‘Multiplikasi tunas dengan metode kultur jaringan jeruk kuok (Citrus nobilis Lour) menggunakan hormon Benzyl Amino Purine (BAP) dan kinetin sebagai rancangan lembar kerja siswa biologi di SMA, JOM FKIP UNRI.

Supriadi, M, Nopsagiarti, T & Ezward, C, 2022 ’Kinetin concentration test on growth castury orange (Citrus microcarpa B.) on medium woody plant medium (WPM)’, In Proceeding of International Conference on Science and Technology, pp. 1-6.

Tuwo, M, Tambaru, E, & Marianty, N, 2022 ‘Respon pertumbuhan biji jeruk keprok Citrus reticulata Blanco pada beberapa teknik sterilisasi. Jurnal Ilmu Alam dan Lingkungan, 13(2):

Yanti, D & Isda, MN, 2021 ‘Shoots induction of nodes (Citrus microcarpa Bunge.) with addition 6-Benzyl Amino Purine (BAP) by in vitro: induksi tunas dari eksplan nodus jeruk kasturi (Citrus microcarpa Bunge.) dengan penambahan 6-Benzyl Amino Purine (BAP) secara in vitro’, Biospecies, vol. 14, no. 1, pp. 53-58.

Yusron, TN, 2020 ‘Respon pertumbuhan eksplan jeruk kasturi (Citrus microcarpa) terhadap pemberian Benzyl Amino Purin (BAP) dan arang aktif pada media MS, Jurnal Agro Indragiri, vol. 5, no. 2, pp. 1-16.

Zulkarnain 2014, Kultur Jaringan Tanaman Solusi Perbanyakan Tanaman Budidaya, Bumi Aksara, Jakarta.

DOI: http://dx.doi.org/10.24014/ja.v16i2.36574