KARAKTERISASI MORFOLOGI DAN UJI ANTIFUNGI ISOLAT JAMUR Trichoderma spp. DARI TANAH GAMBUT TERHADAP PATOGEN PADA JARAK KEPYAR (Ricinus communis L.)

Nur Aini, Atria Martina

Abstract


Castor oil (Ricinus communis L.) is an oil producing commodities that plays a role in meeting industrial and economic needs. Infection with the pathogen fungi caused a decrease in the production of castor oil. Biological control using Trichoderma spp. has been widely used. Trichoderma spp. known as one of the benefical biofungicide because it has high antagonistic properties inhibiting the growth pathogenic fungi. The purpose of this study was to obtain isolates and characters as well antifungal test for Trichoderma spp. isolated from peat soil to control pathogen Aspergillus sp. on castor been (Ricinus communis L.). Trichoderma spp. isolated from peat soil in Meranti Islands, Riau. The antagonistic activity the isolates against pathogen Aspergillus sp. was studied in vitro using dual culture and in vivo assay. In this study, six isolates of Trichoderma spp have isolated. Trichoderma sp. GBA5 has the highest growth rate on PDA and TSM medium. Trichoderma sp. GBA3 and Trichoderma sp. GBA4 were most effective in percentage inhibition of mycelial growth of test pathogen i.e 84,55%  and 83,71% respectively. In in vivo assay, only Trichoderma sp. GBA5 inhibited pathogen with the percent infected castor capsule was 51.66%.


Keywords


Antijamur, Aspergillus sp., jarak kepyar, tanah gambut, Trihoderma spp.

Full Text:

PDF

References


Basetto MA, Chagas HA, Rosa DD, Zanotto MD, Furtado EL. 2008. First report of Rhizoctonia solani AG4 HG-II attacking castor bean plants (Ricinus communis) in Brazil and evaluation of two castor bean cultivars for resistance to damping-off. Australian Plant Disease Notes. 3: 121-123.

Berlian I, Setyawan B, Hadi H. 2013. Mekanisme Antagonisme Trichoderma spp. Terhadap Beberapa Patogen Tular Tanah. Warta Perkaretan. 32(2): 74 – 82.

Berlian I, Sindu A, Endang P. 2016. Isolasi, Identifikasi dan Antagonisme In Vitro Isolat Trichoderma spp. Asal Kebun Karet Blimbing, Pekalongan, Jawa Tengah. Jurnal Penelitian Karet. 34(2): 201-212.

Dawar S, Sumaira K, Marium T. 2014. Seed Borne Mycloflora of Castor Been (Ricinus communis L.) From Pakistan. Pak. J. Bot. 46(4): 1485-1488.

Dwiastuti ME, Fajri MN, Yunimar. 2015. Potensi Trichoderma spp. Sebagai Agens Pengendali Fusarium spp. Penyebab Penyakit Layu pada Tanaman Stroberi (Fragria x ananassa Dutch.). J. Hort. 25(4): 331-339.

Gusnawaty HS, Taufik M, Triana L, Asniah D. 2014. Karakterisasi Morfologis Trichoderma spp. Indigenus Sulawesi Tenggara. Jurnal Agroteknos. 4(2) : 88-94.

Hewedy OA, Khalid SAL, Mahmoud FS, Ashwag Shami, Fawziah MA, Rasha M El-Meihy. 2020. Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions. Biology. 9(189): 1-20.

Howell CR. 2003. Mechanisms Employed by Trichoderma species in the Biological Control of Plant Diseases: The History and Ecolution of Current Concepts. Plant Disease. 87(1): 4-10.

Jin X, Guo L, Jin B, Zhu S, Mei X, Wu J, He X. 2020. Inhibitory Mechanism of 6-Pentyl-2H-pyran-2-one Secreted by Trichoderma atriviride T2 Against Cylindrocarpon destrucants. Pesticide Biochemistry and Physiology, 170, 104683.

Kaunang RA, Assa BH, Montong VB. 2018. Uji Antagonisme Trichoderma spp. Terhadap Phytophthora Palmivora Penyebab Penyakit Gugur Buah Kelapa. In Cocos. 1(3).

Khan R, Najeeb S, Hussain S, Xie B, Li Y. 2020. Bioactive Secobdary Metabolites from Trichoderma spp. Against Phytopathogenic Fungi. Microorganism. 8(2): 817.

Konstantinou S, Veloukas T, Leroch M, Menexes G, Hahn M, Karaoglanidis G. 2015. Population Structure, Fungicide Resistance Profile, and sdhB Mutation Frequency of Botrytis cinerea From Strawberry and Greenhouse-grown Tomato in Greece. Plant Dis. 99: 240-248.

Muksin R, Rosmini, Panggeso J. 2013. Uji Antagonisme Trichoderma sp. terhadap Jamur Patogen Alternaria porri Penyebab Penyakit Bercak Ungu pada Bawang Merah Secara In-vitro. E-Journal Agrotekbis. 1(2) : 140–144.

Mamza WS, Zarafi AB, Alabi O. 2010. Fusarium pallidoroseum L. on castor (Ricinus communis) in Samaru, Nigeria. Archives of Phytopathology and Plant Protection. 43(7-9): 871-874.

Muhibuddin A, Setiyowati EM, Sektiono AW. 2021. Mechanism Antagonism of Trichoderma viridae Against Several Types of Pathogenes and Production of Secondary Metabolites. Agrosaintifika: Jurnal Ilmu-ilmu Pertanian. 4: 243-253.

Novianti Dewi. 2018. Perbanyakan Jamur Trichoderma sp. pada Beberapa Media. Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam. 15(1): 35-44.

Octriana L. 2011. Potensi Agen Hayati alam Menghambat Pertumbuhan Phytium sp. secara In Vitro. Buletin Plasma Nutfah. 17(2): 138-142.

Purwandriya F. 2016. Kemampuan Trichoderma sp. dalam Menghambat Curvularia lunata Penyebab Penyakit Bercak Daun pada Tanaman Nenas (Ananas comosus L Merr.) Skripsi. Fakultas Pertanian. Universitas Lampung.

Purwanti Sari, Rini BH. 2009. Uji Antagonisme Jamur Patogen Phytophthora infestans penyebab penyakit busuk Daun dan Umbi Tanaman Kentang Dengan Menggunakan Trichoderma spp. Isolat Lokal. BIOMA. 11(1): 24-32.

Puspita F, Isna RD, Dermala Sari. 2019. Screening of Fungi ftom Oil Palm Rhizosphere in Peat Soils and the Potential as Biological Agents Against Ganoderma bonisense. Indonesian Journal of Agricultural Research. 2(2): 97-109.

Rahmadani S, Salamiah, Helda OR. 2021. Pengujian Dua Belas Isolat Trichoderma spp. Asal Lahan Rawa Pasang Surut untuk Menghambat Fusarium oxysporum Penyebab Penyakit Moler pada Tanaman Bawang Merah (Allium ascalonicum L.). Ptoteksi Tanaman Tropika. 4(02): 330-336.

Ratnasari JD, Isnawati, Ratnasari E. 2014. Uji Antagonis Agens Hayati Terhadap Jamur Cercospora musae cause disease Sigatoka by In Vitro. LenteraBio. 3(2): 129-135.

Roman DL, Denisa IV, Madalina Filip, Vasile O, Adriana I. 2021. Effects of Triazole Fungicides on Soil Microbiota and on the Activities of Enzymes Found in Soil: A Review. Agriculture. 11(893): 1-18.

Safitri N, Martina A, Roza RM. 2019. Uji Antagonis Cendawan Isolat Lokal Riau Terhadap Beberapa Cendawan Patogen Pada Tanaman Budidaya. Al- Kauniyah: Jurnal Biologi. 12(2): 124-132.

Samson RA, J Houbraken, U Tharane, JC Frisvad, B Andersen. 2010. Food and Indoor Fungi. CBS-KNAW Fungal Biodiversity Centre, The Netherlands.

Saputra S. 2020. Uji Efektivitas Jamur Trichoderma spp. dalam Mencegah Penyakit Layu Fusarium (Fusarium oxysporum) Pada Tanaman Bawang Merah dengan Kerapatan Konidia yang Berbeda. [Skripsi]. Medan: Fakultas Pertanian, Universitas Muhammadiyah Sumatera Utara.

Saputra R, Fifi P, Anthony H, Irfandri, Eka S. 2022. Morphological Characterization of Trichoderma spp. Isolated From the Oil Palm Rhizosphere in Peat Soils and its Potential as a Biological Control for Ganoderma sp. in vitro. Jurnal Ilmiah Pertanian. 19(2): XX-XX.

Shahid Mohd, Anuradha S, Mukesh S, RP Mishra, SK Biswas. 2011. Effect of Temperature, pH, amd Media For Growth and Sporulation of Trichoderma longibrachiatum and Self Life Study in Carrier Based Formulations. Ann. Pl. Protec. Sci. 19(1): 147-149.

Soares DJ. 2012. Gray Mold Of Castor: A Review. Plant pathology, 219-240.

Watanabe T. 2002. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species Second Edition. CRC PRESS, Washington, D.C.

Yamuna C, P Kishore Varma, RD Prasad. 2021. Management Of Castor Gray Mold Using Trichoderma Species. Journal of Entomology and Zoology Studies. 10(1): 111-115.

Zin Nur A, Badaluddin Noor A. 2020. Biological Functions Of Trichoderma spp. For Agriculture Applications. Annals of Agricultural Sciences. 65: 168–178.




DOI: http://dx.doi.org/10.24014/ja.v14i2.18479

Refbacks

  • There are currently no refbacks.


 

Alamat : Jalan H.R Soebrantas KM 15 Panam, Pekanbaru, Riau.

Email : jurnalagroteknologi@yahoo.com


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

View My Stats