REVIEW: PEMANFAATAN BROMELAIN PADA BEBERAPA PANGAN LOKAL INDONESIA

Warsono El Kiyat, Kevin Reynaldo, Jeremiah Irwan

Abstract


Bromelain is one of the protease that can be produced from all of parts of the pineapple plants (Ananas comosus). It has potential to improve quality of local Indonesian food such as tempeh gembus, virgin coconut oil (VCO), and dumbo catfish based fish sauce (DCBFS). Tempeh gembus is a traditional food that has unique characteristics especially in its taste because it contains amino acids. VCO attracts consumers because of its health aspect. Its raw materials are available in Indonesia. DCBS  is a product of fermented fish with salt that has a distinctive taste and is produced in Indonesia. This study aimed to analyze the effect of bromelain on local Indonesian food and its applications. The result showed that the use of bromelain in local Indonesian food could improve the quality and nutritional value of local food. Bromelain can be used in local food like tempeh gembus, virgin coconut oil, and fish sauce from dumbo catfish. The use of bromelain in tempeh gembus could  increase both of the water-soluble nitrogen level and the pH value. However, the addition of bromelain in the production of virgin coconut oil and fish sauce from dumbo catfish could increase the yield of theirs. .


Keywords


Bromelain; fish sauce; local food; protease; tempeh gembus; virgin coconut oil

Full Text:

PDF

References


Adawiyah, D.R., Soekarto, T.S., Hariyadi, P., 2012. Fat hydrolysis in a food model system: effect of water activity and glass transition. Int. Food Res. J. 19, 737–741.

Anwar, C., Salma, Z., 2016. Perubahan rendemen dan mutu virgin coconut oil (VCO) pada berbagai kecepatan putar dan lama waktu sentrifugasi. J. Teknotan 10, 51–60.

Arshad, I.Z.M., Amid, A., Yusof, F., Jaswir, I., Ahmad, K., Loke, S.P., 2014. Bromelain : an overview of industrial application and purification strategies. Appl. Micriobiology Biotechnol. 98, 7283–7297. https://doi.org/10.1007/s00253-014-5889-y

Benucci, I., Liburdi, K., Maria, A., Garzillo, V., Esti, M., 2011. Bromelain from pineapple stem in alcoholic – acidic buffers for wine application. Food Chem. 124, 1349–1353. https://doi.org/10.1016/j.foodchem.2010.07.087

Bhattacharya, R., Bhattacharyya, D., 2007. Preservation of natural stability of fruit “bromelain” from. J. Food Biochem. 33, 1–19.

Blanco, A., Blanco, G., 2017. Enzymes, Medical Biochemsitry. Academic Press, Cambridge, USA.

Chaisakdanungull, C., Theerakulkait, C., Wrolstad, R.D., 2007. Pineapple Juice and Its Fractions in Enzymatic Browning Inhibition of Banana [ Musa ( AAA Group ) Gros Michel ]. J. Agric. Food Chem. 55, 4252–4257.

Dewi, K., Karnila, R., Loekman, L., 2016. Pengaruh Penambahan Crude Enzim Bromelin Berbeda Terhadap Kualitas Kecap Ikan Lele Dumbo (Clarias gariepinus). Universitas Riau.

Elavarasan, K., Kumar, V.N., Shamasundar, B.A., 2014. Antioxidant and Functional Properties of Fish Protein Hydrolysates from Fresh Water Carp (Catla catla) as Influenced by the Nature of Enzyme. J. Food Process. Preserv. 38, 1207–1214. https://doi.org/10.1111/jfpp.12081

Feijoo-siota, L., Villa, T.G., 2011. Native and Biotechnologically Engineered Plant Proteases with Industrial Applications. Food Bioprocess Technol. 4, 1066–1088. https://doi.org/10.1007/s11947-010-0431-4

Florence, A., Attwood, D., 2011. Peptides, Proteins and Other Biopharmaceuticals, in: Physicochemical Principles of Pharmacy. Pharmaceutical Press Publishing, London, hal. 451–476.

Gerelt, B., Ikeuchi, Y., Suzuki, A., 2000. Meat Tenderization by Proteolytic Enzymes After Osmotic Dehydration. Meat Sci. 56, 311–318.

Hage, D.S., Anguizola, J.A., Bi, C., Li, R., Matsuda, R., Pfaunmiller, E., Vargas, J., Zheng, X., 2013. Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments. J. Pharm. Biomed. Anal. 69, 93–105. https://doi.org/10.1016/j.jpba.2012.01.004.PHARMACEUTICAL

Himonides, A.T., Taylor, A.K.D., Morris, A.J., 2011. A Study of the Enzymatic Hydrolysis of Fish Frames Using Model Systems. Food Nutr. Sci. 2, 575–585. https://doi.org/10.4236/fns.2011.26081

Hou, H., Li, B., Zhao, X., Zhang, Z., Li, P., 2011. Optimization of enzymatic hydrolysis of Alaska pollock frame for preparing protein hydrolysates with low-bitterness. LWT - Food Sci. Technol. 44, 421–428. https://doi.org/10.1016/j.lwt.2010.09.009

Kong, X., Zhou, H., Qian, H., 2007. Enzymatic hydrolysis of wheat gluten by proteases and properties of the resulting hydrolysates. Food Chem. 102, 759–763. https://doi.org/10.1016/j.foodchem.2006.06.062

Liang, H., Li, M., Shi, M., Liao, A., Wu, R., 2012. Study on the Stability of Fruit Bromelain. Adv. Mater. Res. 421, 19–22. https://doi.org/10.4028/www.scientific.net/AMR.421.19

Mansor, T., Che Man, Y., Shuhaimi, M., Abdul, A.M.J., Ku, N.F.K.M., 2012. Physicochemical properties of virgin coconut oil extracted from different processing methods 19, 837–845.

Marina, A.M., Che Man, Y.B., Amin, I., 2009a. Virgin coconut oil : emerging functional food oil. Trends Food Sci. Technol. 20, 481–487. https://doi.org/10.1016/j.tifs.2009.06.003

Marina, A.M., Che Man, Y.B., Nazimah, A.H., Amin, I., 2009b. Chemical Properties of Virgin Coconut Oil. J. Am. Oil Chem. Soc. 86, 301–307. https://doi.org/10.1007/s11746-009-1351-1

Nurhidayah, N., Masriany, M., Mashuri, M., 2013. Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Batang Nanas (Ananas comosus) Berdasarkan Variasi pH. BioGenesis 1, 116–122.

Onsaard, E., Vittayanont, M., Srigam, S., Mc Clement, D., 2005. Properties and stability of oil in water emulsions stabilized by coconut skim milk protein. J. Agric. Food Chem. 53, 5747–5753.

Osawaa, C.C., Goncalvesa, L.A.G., Ragazzib, S., 2007. Correlation between free fatty acids of vegetable oils evaluated by rapid tests and by the official method. J. Food Compos. Anal. 20, 523–528. https://doi.org/10.1016/j.jfca.2007.02.002

Palupi, N.W., Windrati, W.S., Tamtarini, T., 2010. The Effect of Enzymatic Hydrolysis on the Properties of Protein Hydrolysate from Paddy Mushroom. Makara, Teknol. 14, 73–76.

Pavan, R., Jain, S., Shraddha, S., Kumar, A., 2012. Properties and therapeutic application of bromelain: A review. Biotechnol. Res. Int. 976203, 1–6. https://doi.org/10.1155/2012/976203

Raghavendra, S.N., Raghavarao, K.S.M.S., 2010. Effect of different treatments for the destabilization of coconut milk emulsion. J. Food Eng. 97, 341–347. https://doi.org/10.1016/j.jfoodeng.2009.10.027

Rahman, M.S., 2010. Food stability determination by macro-micro regio concept in the state diagram and by defining a critical temperature. J. Food Eng. 99, 402–416.

Salahudin, F., Supriyatna, N., 2014. Ekstraksi Minyak Kelapa secara Fermentasi untuk Mempertahankan Mutu Asam Lemak Rantai Sedang. Biopropal Ind. 5, 23–28.

Sangjindavong, M., Mookdasanit, J., Wilaipun, P., Chuapoehuk, P., Akkanvanitch, C., 2009. Using Pineapple to Produce Fish Sauce from Surimi Waste. Kasetsart J. (Natural Sci. 43, 791–795.

Savitri, R., 2011. Aplikasi Proses Hidrolisis Enzimatis dan Fermentasi dalam Pengolahan Condiment Kupang Putih (Corbula faba H.). Institut Pertanian Bogor.

Sebayang, F., 2006. Pengujian stabilitas enzim bromelin yang diisolasi dari bonggol nanas serta imobilisasi menggunakan kappa karagenan. J. Sains Kim. 10, 20–26.

Silaban, R., Manullang, R.S., Hutapea, V., 2010. Pembuatan Virgin Coconut Oil (VCO) Melalui Kombinasi Teknik Fermentasi dan Enzimatis Menggunakan Ekstrak Nenas. Universitas Negeri Medan.

Sree, P.P.S., Jayakumar, K., Mathai, V., Chintu, S., Sarath, B.K., 2012. Immobilization and Kinetic Studies of Bromelain: A Plant Cysteine Protease From Pineapple (Ananas Comosus) Plant Parts. Int. J. Med. Heal. Sci. 1, 10–16.

Subagio, A., Hartanti, S., Windrati, W., Unus, U., Fauzi, M., Herry, B., 2002. Kajian Sifat Fisikokimia dan Organoleptik Hidrolisat Tempe Hasil Hidrolisis Protease. J. Teknol dan Ind. Pangan 13, 204–210.

Sunarsih, L., 2008. Uji Efektivitas Ekstrak Buah Nanas (Ananas comosus L. Merr) Muda Varietas Queen Terhadap Mortalitas Cacing Parasit (Ascaridia Galli) Secara In Vitro. Universitas Muhammadiyah Malang.

Susanto, T., 2012. Kajian metode pengasaman dalam proses produksi minyak kelapa ditinjau dari mutu produk dan komposisi asam amino blondo. J. Din. Penelit. Ind. 23, 124–130.

Susi, S., 2012. Komposisi Kimia dan Asam Amino pada Tempe Kacang Nagara (Vigna unguiculata ssp. cylindrica). Agroscientiae 19, 28 – 36.

Utami, D.P., Pudjomartatmo, P., Nuhriawangsa, A.M.P., 2011. Manfaat Bromelin dari Ekstrak Buah Nanas (Ananas comosus L. Merr) dan Waktu Pemasakan untuk Meningkatkan Kualitas Daging Itik Afkir. Sains Peternak. 9, 82–87.

Watanabe, M., Watanabe, J., Sonoyama, K., Tanabe, S., 2000. Novel method for producing hypoallergenic wheat flour by enzymatic fragmentation of the constituent allergens and its application to food processing. Biosci. Bitechnology, Biochem. 64, 2663–2667.

Wijaya, J.C., Yunianta, Y., 2015. Pengaruh Penambahan Enzim Bromelin Terhadap Sifat Kimia dan Organoleptik Tempe Gembus (Kajian Konsentrasi dan Lama Inkubasi dengan Enzim). J. Pangan dan Agroindustri 3, 96–106.

Wijayanti, I., Romadhon, R., Rianinsih, L., 2016. Karakteristik Hidrolisat Ikan Bandeng (Chanos chanos Forsk) dengan Konsentrasi Enzim Bromelin yang Berbeda. J. Saintek Perikan. 11, 129–133.




DOI: http://dx.doi.org/10.24014/ja.v10i1.6515

Refbacks

  • There are currently no refbacks.


 

Alamat : Jalan H.R Soebrantas KM 15 Panam, Pekanbaru, Riau.

Email : jurnalagroteknologi@yahoo.com


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

View My Stats