ISOLASI DAN UJI POTENSI BAKTERI DIAZOTROF NON SIMBIOTIK ASAL TANAH KEBUN KELAPA SAWIT DENGAN APLIKASI TANDAN KOSONG DAN LIMBAH CAIR PABRIK KELAPA SAWIT

Huryatul Islam, Nelvia Nelvia, Delita Zul

Abstract


This study aimed was to isolate non-symbiotic N-fixation bacteria from oil palm plantations fertilized with empty fruit bunch (EFB) and palm oil mill effluent (POME) and to analyze their potency. The soil samples were taken by purposive sampling method from 4 different treatments are mixed application (EFB + POME), EFB application, POME application and without application (C), and each location was taken 3 times. Isolation of non-symbiotic N-fixation bacteria was perfomed by using NFb and Ashby's media. The parameters observed were bacterial cells number, number of isolates and their potency. The cells number of non-symbiotic N fixation bacteria at the application sites Mixed> POME application> EFB application> C. A total of 18 isolates were isolated and potentially as a nitrogen fixing bacteria characterized by the ability of isolates to change the color of the NFb medium from green to blue and form pellicle. The highest potential isolates are NFBC232, NFBK21, and NFBC33.


Keywords


Non symbiotic N fixing bacteria, empty fruit bunch, palm oil mill effluent.

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References


Agustian, Syafei R., Maria, L. 2012. Keragaman Bakteri Penambat N Pada Rhizosfer Titonia (Titonia diversifolia) yang Tumbuh Pada Tanah Masam Ultisol. Jurnal Solum. Vol 9 No.2: 98-105.

Baldani, JL., Reis, VM., Videira, SS., Boddey, LH., Baldani, VLD. 2014. The Art of Isolating Nitrogen Fixing Bacteria from Non-Leguminous Plant using N Free Semi Solid Media: a Particular Guide for Microbiologists. Plant Soil D0I 10.1007/s11104-014-218-6.

Bishnu, Saha, T., Mazumdar, D., Chakrabarti, K., Chakraborty, A. 2008. Assasement of the Impact of Pesticide Residues on Microbiological and Biochemical Parameters of Tea Garden Soils in India, Journal of Enviromental Science and Healt. 43 (8):723-731.

Danapriatna, N. 2010. Biokimia Penambatan Nitrogen oleh Bakteri Non Simbiotik. Jurnal Agribisnis dan Pengembangan Wilayah. Vol.1 No.2.

Firrani, M. 2011. Isolasi dan Uji Kemampuan Bakteri Endofit Diazotrof yang Memfiksasi Nitrogen Bebas pada Akar Kelapa Sawit (Elaeis guineensis Jaqc.). Departemen Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam. USU. Medan.

Hartono and Jumadi, O. 2014. Seleksi dan Karakterisasi Bakteri Penambat Nitrogen Non Simbiotik Pengekskresi Amonium Pada Tanah Pertanaman Jagung (Zea mays L.) dan Padi (Oryza sativa L.) Asal Kabupaten Barru Sulawesi Selatan Indonesia. Jurnal Sainsmat. Vol.3. No.2:143-153.

Handayanto, E., Hairiah K. 2007. Biologi Tanah: Landasan Pengelolaan Tanah Sehat. Pustaka Adipura. Yogyakarta.

Harran, S., Ansori N. 1992. Bioteknologi Pertanian Bogor: Pusat Antar Universitas Bioteknologi Institut Pertanian Bogor.

Hosaain, MM., Iffat, J., Salina, A. Md. NR,, Badier, SMR. 2015. Isolation and Identification of Azospirillum Isolates from Different Paddy Fields of North Bengal. Indian Journal of Research Pharmacy and Biotechnology. Vol. 3(1):74-80.

Husen, E. 2007. Pengambilan Contoh Tanah untuk Analisis Mikroorganisme dalam R.D.M. Simanungkit, Suriadikarta, Saraswati, E. Husein, Balitbang Sumberdaya Lahan Pertanian, Depertemen Pertanian. Jakarta.

Jolly, SN., Shanta, NA., Zahed, UMK. 2010. Quantification of Heterotrophic Bacteria and Azospirillum from the Rhizosphere of Taro (Colocasia esculenta L. Schoot.) and the Nitrogen Fixing Potensial of Isolated Azospirillum. International Journal of Botany 6(2):117-121.

Karina, A.I. 2016. Isolasi dan Identifikasi Bakteri Penambat Nitrogen, Pelarut Fosfat, dan Bakteri Pendegradasi Selulosa pada Tanah Bekas Tanaman Bawang (Allium cepa L.) yang diberi Biofertilizer. Fakultas Sains dan Teknologi. Universitas Airlangga.

Kizilkaya, R. 2009. Nitrogen Fixation Capacity of Azotobacter spp. Strains Isolated from Soils in Different Ecosystems and Relationship Between them and the Microbiological Properties of Soils. Journal of Enviromental Biology. 30(1):73-82.

Lay, L.B.W. 1994. Analisis Mikroorganisme di Laboratorium. PT Raja Grapindo Persada, Jakarta.

Madigan, M.T., Martinko, J.M., Stahl, D.A., Clark, D.P. 2012. Brock Biology of microorganism. Edisi ke-13. Benjamin Cummings. San Francisco.

Metasari k. 2011. Eksplorasi Bakteri Penambat Nitrogen Non Simbiotik dari Tanah Kawasan Mangrove Wonorejo Surabaya. Universitas Airlangga.

Novita D. 2013. Pengaruh Pupuk Terhadap Sifat Kimia Tanah dan Populasi Mikrob Rizosfer Tanaman Kilemo (Litsea cubeba Pers). Departemen Ilmu Tanah dan Sumberdaya Lahan Fakultas Pertanian Bogor. IPB. Bogor.

Oliveira, ALM., Canuto, EL., Silva, EE., Reis, VM., Baldani, Jl. 2014. Survival of Endophytic Diazotrophic Bacteria in Soil Under Different Moisture Levels. Journal Brazilian Microbiology 35:295-299.

Pelczar, M. J dan Chan, E. C. S. 2010. Dasar-Dasar Mikrobiologi. Edisi 2, Terjemahan Hadioetomo, R.S., Imas, T., Tjitrosomo, S.S dan Angka, S.L, UI-Press. Jakarta.

Raffi, MMD., Charyulu, PBBN. 2012. Nitrogen Fixation by the Native Azotospirillum spp. Isolate from Rhizosphere and Non-Rhizosphere of Foxtail Millet. Asian Journal Biological and Life Science. 1:213-218.

Schlegel, HG. 1994. Mikrobiologi Umum. Ed ke-6. Terjemahan dari Microbiology 6st editing, oleh R.M Tedjo Baskoro. 1984. Yogyakarta: Gadjah Mada. University Press.

Simanungkalit, R.D.M., Saraswati, R., Hastuti, R.D., dan Edi E 2006. Bakteri Penambat Nitrogen. hlm 113-140 dalam Simanungkalit, R.D.M., Suriadikarta, D.A., Saraswati, R.D., Setyorini dan Hartatik, W. Pupuk Organik dan Pupuk Hayati. Balai Besar Litbang Sumberdaya Lahan Pertanian Bogor.

Silva, KD., Rafaela, SAN., Adriana, SL., Alexandra, B., Fatima, MDSM. 2011. Density and Diversity of Diazotrophic Bacteria Isolated from Amazonian Soils using N-Free Semi-Solid Media. Science Agriculture. 68(5):518-525.

Susilowati, DN., Saraswati, R., Hastuti, RD., Yuniarti, E. 2007. Peningkatan Serapan N pada Kedelai yang Diinokulasi Bakteri Diazotrof Endofit di Medium Vermiculit. Jurnal Tanah Iklim, 26:41-46.

Tarigan, RS., Jamilah, I., Elimasni. 2013. Seleksi Bakteri Penambat Nitrogen dan Penghasil Hormon IAA (Indole Acetic Acid) dari Rizosfer Tanah Perkebunan Kedelai (Glycine max L.). Sin Biology. Vol. 1 (2):42-48.

Waluyo, L. 2010. Teknik dan Metode Dasar Dalam Mikrobiologi. Universitas Muhammadiyah. Malang.

Widawati, S. 2015. Uji Bakteri Simbiotik dan Nonsimbiotik Pelarut Ca vs P dan Efek Inokulasi Bakteri Pada Anakan Turi (Sesbania grandiflora L.Pers.). Jurnal Biologi Indonesia. Vol. 11 (2): 295-307.

Widhiastuti, R., Suryanto, D., Muklis., Wahyuningsih, H. 2006. Pengaruh Pemanfaatan Limbah Cair Pabrik Pengolahan Kelapa Sawit Sebagai Pupuk terhadap Biodiversitas Tanah. Jurnal Ilmiah Pertanian Kultura. Vol. 41. N0 1.

Widyastuti, H., Siswanto dan Suharyanto. 2010. Karakterisasi dan Seleksi Beberapa Isolat Azotobacter sp. untuk Meningkatkan Perkecambahan Benih dan Pertumbuhan Tanaman. Buletin Plasma Nutfah. Vol. 16(2):160-167.

Winarti, S and Neneng, L. 2013. Pengaruh Pemberian Limbah Kelapa Sawit Terhadap Sifat Fisika, Kimia dan Biologi Tanah pada Lahan Kritis Eks Penambangan Emas. Jurnal Agripeat. Vol. 14. N0 2.




DOI: http://dx.doi.org/10.24014/ja.v9i2.4508

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